云南云秃蝗不同地理种群染色体多态性分析  

Chromosome Polymorphism of Three Populations of the Grasshopper Yunnanacris yunnaneus (Ramme)

作　　者：李金玲 张晓明[1] 张睿[1] 柳青[1,2] 欧晓红 LI Jinling;ZHANG Xiaoming;ZHANG Rui;LIU Qing;OU Xiaohong(Plant Protection College, Yunnan Agricultural University, National Key Laboratory for Conservation and Utilization of Biological Resom-ces in Yunnan, Kunming 650201, China;Department of Resources and Environmental Sciences, Baoshan College, Baoshan 678000, China;Key Laboratory of Forest Disaster Warning and Control in Yunnan Province, College of Forestry, Southwest Forestry University, Kunming 650224, China)

机构地区：[1]云南农业大学植物保护学院、云南生物资源保护与利用国家重点实验室,云南昆明650201 [2]保山学院资源环境学院,云南保山678000 [3]西南林业大学林学院,云南省森林灾害预警与控制重点实验室,云南昆明650224

出　　处：《云南农业大学学报（自然科学版）》2018年第1期34-40,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金项目(31400351,30760033)

摘　　要：【目的】云南云秃蝗[Yunnanacris yunnaneus(Ramme)]是中国特有种,为了解云南云秃蝗不同地理种群的染色体特征。【方法】采用常规的液氮冰冻压片法和BSG显带法对云南云秃蝗3个地理种群的染色体核型和C带带型进行了研究。【结果】云南云秃蝗3个地理种群的染色体数目都为2n(♂)=21;染色体类型多样,具有端、近端、中及亚中着丝粒染色体;性别决定机制均为XO型。染色体分组形式基本是3~4对大型、3~6对中型、1~3对小型染色体,性染色体X属大型染色体。C带分析显示:云南云秃蝗C带带型简单,仅具有着丝粒带和端带,但带纹数量在3个种群间具有差异,最多的有21条;种的平均异染色质含量为20.99%。【结论】3个种群在染色体C带核型和外部形态两个方面差异显著,结果对进一步研究云南云秃蝗种群分化提供了重要的参考依据。[Purpose]Yunnanacris yunnaneus（Ramme） is an endemic species of China, in order to understand the cytogenetic characteristics of different populations of Y. yunnaneus.[Method]The chromosome karyotype and C-band patterns of the three geographic populations of Y. yunnaneus have been studied using squashing and BSG chromosomal banding technique in this paper.[Results]Three populations of Y. yunnaneus have a diploid chromosome number of 2 n（♂）=21, with an XO sex-determining mechanism and a diverse chromosome type consisting of telocentric, subtelocentric,metacentric and submetacentric chromosomes. The autosomes can be divided into 3-4 large, 3-6 medium and 1-3 short groups according to their relative length, the X chromosome is the large one in size. C-banding reveals that the three populations have a simple type only with centromeric and terminal C-band, and the numbers are different among populations, and the most having 21 C-bands.The average value of heterochromatic content in this species is 20.99% .[Conclusion]The three populations show obvious differences in the two aspects of C-band karyotype and external morphology, and which provide an important references for further study of the population differentiation of this species.

关 键 词：云南云秃蝗 地理种群 染色体 C带核型 

分 类 号：Q969.265.1[生物学—昆虫学]