3种试剂盒提取烤后烟叶DNA的效果比较  

Comparison of the DNA Extraction Effects of Tree Kits on the Tobacco after Baking

作　　者：陆铮铮[1] 李芳 LU Zhengzheng;LI Fang(College of Life Sciences, Zunyi Normal College, Zunyi 563006, Chin)

机构地区：[1]遵义师范学院生物与农业科技学院,贵州遵义563002

出　　处：《云南农业大学学报（自然科学版）》2018年第1期172-175,共4页Journal of Yunnan Agricultural University:Natural Science

基　　金：贵州省科技支撑计划农业攻关项目[黔科合NY(2013)3020]

摘　　要：【目的】筛选出简单、快速及高效的烤后烟叶DNA提取试剂盒。【方法】选用3种不同的DNA提取试剂盒提取烤后烟叶DNA,通过比较DNA的纯度和浓度、实时荧光定量PCR扩增Ct值以及凝胶电泳图谱分析的方法,综合评价DNA提取效果。【结果】AxyPrep基因组DNA小量试剂盒所提取的DNA,其纯度和浓度较高,用时短,DNA质量好,适用于小量DNA的提取;磁珠法植物基因组DNA试剂盒提取DNA,其浓度和纯度虽然略高于前者,但用时很长,比较适用于批量DNA的提取;Gene Pure新型植物基因组DNA快速提取试剂盒提取DNA,其纯度高、但浓度低。【结论】烤后烟叶DNA的提取推荐使用AxyPrep基因组DNA小量试剂盒。[Purpose]In order to select a kit of simple, fast and efficient to extract the tobacco after baking DNA, we had selected 3 different kits to extract the tobacco after baking DNA.[Method]By comparing the purity and concentration of DNA, quantitative real-time PCR of Ct and gel electrophoresis, we had evaluated the effect.[Results]Using the AxyPrep DNA kit to extract DNA, the purity and concentration of DNA were good, short time to use, the quality was good,apply to extract a little DNA; Using the Magnetic bead DNA kit to extract DNA, the purity and concentration of DNA were slightly higher than the former, but long time to use, suitable to extract lot of DNA; Using the Gene Pure DNA kit to extract DNA, the purity of DNA were high, but the concentration of DNA were low.[Conclusion]We recommend the use of the AxyPrep DNA kit to extract the tobacco after baking DNA.

关 键 词：烤后烟叶 DNA提取试剂盒 实时荧光定量PCR 凝胶电泳 

分 类 号：S572.035.3[农业科学—烟草工业]