不同大小剪切力对血管内皮细胞IL-8及ET-1表达的影响  

Effect of different shear stress on the expression of IL-8 and ET-1 in vascular endothelial cells

作　　者：范涛[1] 于晓东[1] 陈春玲[1] Fan Tao;Yu Xiaodong;Chen Chunling(Department of Anesthesiology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Chin)

机构地区：[1]新疆医科大学第一附属医院麻醉科,乌鲁木齐830054

出　　处：《中国医师杂志》2018年第5期667-671,共5页Journal of Chinese Physician

基　　金：国家自然科学基金(U1603129)~~

摘　　要：目的探讨不同大小剪切力对血管内皮细胞白介素-8(IL-8)及内皮素-1(ET-1)表达的影响。方法采用脐静脉内皮细胞株(ECV-304)作为研究细胞,使用自制的剪切力调控装置调节出5 dyne/cm^2和10 dyne/cm^2的剪切力,其中5 dyne/cm^2的剪切力作为低剪切力组,10 dyne/cm^2的剪切力作为高剪切力组,另选用未施加剪切力的ECV-304作为对照组。分析三组在五个时间点(30、60、120、300、420 min)的IL-8和ET-1水平,并采用Pearson相关分析方法分析IL-8和ET-1水平的相关性。结果三组的IL-8水平一直呈上升趋势。在30、60、120 min三个时间点,三组的IL-8水平整体比较差异无统计学意义(P>0.05)。在300、420 min两个时间点,低剪切力组的IL-8水平高于对照组和高剪切力组,高剪切力组的IL-8水平高于对照组(P<0.05)。对照组和低剪切力组的ET-1水平一直呈上升趋势,高剪切力组的JET-1水平在前120 min持续上升,但在此后开始持续下降。在30、60、120 min三个时间点,三组的ET-1水平整体比较差异具有统计学意义(P<0.05),高剪切力组在30、60、120 min三个时间点的ET-1水平均高于对照组,在30、60 min两个时间点的ET-1水平高于低剪切力组(P<0.05);低剪切力组在60、120 min两个时间点的ET-1水平高于对照组(P<0.05);在300、420 min两个时间点,三组的ET-1水平整体比较差异无统计学意义(P>0.05)。Pearson相关分析显示,IL-8与ET-1的表达存在正相关关系(r=0.384,P=0.031)。结论剪切力可对血管内皮细胞IL-8、ET-1的表达造成影响,其中5 dyne/cm^2的剪切力能够更高效地增加IL-8水平,10 dyne/cm^2的剪切力能够更明显地增加ET-1水平,但可能会对血管内皮细胞造成损伤。Objective To investigate the effect of different shear stress on the expression of Interleukin -8 （IL-8） and endothelin -1 （ET-1） in vascular endothelial cells. Methods Umbilical vein endothelial cell line （ ECV-304 ） was used as the research cell. The shear stress of 5 dyne/cm^2 and 10 dyne/cm^2 was regulated by the self-made shearing stress control device. The shear stress in were maintained at 0,5 dyne/cm^2 and 10 dyne/cm^2 respectively in the control group, the low shear stress group and the high shear stress group. The effects on the levels of IL-8 and ET-1 were analyzed between the three groups at five time points of 30 min,60 rain,120 min,300 min,420 rain. Pearson correlation analysis was used to analyze the correlation between IL-8 and ET-1. Results The level of IL-8 in the three groups has been on the rise. At the three time points of 30 rain ,60 min and 120 min, there was no statistically significant difference in IL-8 level between the three groups （ P 〉 0. 05 ）. At the two time points of 300 rain and 420 rain, the difference of the IL-8 level in the three groups was statistically different （ P 〈 0. 05 ） , At the two time points of 300 min and 420 min, the IL-8 level in the low shear stress group was higher than that in the control group and the high shear group, the IL-8 level in the high shear group was higher than that in the control group （ P 〈 0. 05 ）.The level of ET-1 in the control group and the low shear group showed an upward trend, and the ET-1 level in the high shear group increased continuously in the former 120 min, but began to decrease continuously since then. At the three time points of 30 min,60 rain and 120 min,the difference of the ET-1 level in the three groups was statistically different （ P 〈 0. 05 ）. The ET-1 level of the high shear group at three time points of 30 min,60 min and 120 min was higher than that of the control group, the level of ET-1 at two time points of 30 min and 60 min was higher than that in low shear stress group （P 〈0.

关 键 词：粘度 内皮 血管/代谢 内皮细胞/代谢 白细胞介素8/代谢 内皮缩血管肽1/代谢 

分 类 号：R614[医药卫生—麻醉学]