新型纳米微流控芯片检测胆囊癌患者外周血CTCs与临床病理及预后的关系  

Relationships between peripheral circulating tumor cells in patients with gallbladder carcinoma de- tected by a novel nano microfluidic chip with clinicopathology and prognosis

作　　者：王晓光 吴斌 倪全法 亓立峰[2] 费建国 钟征翔 Wang Xiaoguang;Wu Bin;Ni Quanfa;Qi Lifeng;Fei Jianguo;Zhong Zhengziang.(Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Jiaxing College of Medicine, Jiaxing 314000, China)

机构地区：[1]嘉兴医学院附属第二医院肝胆外科,314000 [2]同济大学医学院生物医学工程和纳米科学研究院

出　　处：《中华肝胆外科杂志》2018年第5期321-324,共4页Chinese Journal of Hepatobiliary Surgery

基　　金：嘉兴市公益性应用技术研究计划（2016AY23053）

摘　　要：目的建立新型纳米微流控芯片检测胆囊癌患者外周血循环肿瘤细胞（CTCs）的方法，探讨其与临床病理、生存预后的相关性。方法收集2014年6月至2017年1月嘉兴医学院附属第二医院收治的51例胆囊癌患者资料，应用新型纳米微流控芯片检测外周血CTCs，通过Kaplan-Meier生存分析，评估外周血CTCs与临床病理的关系及其对预后的影响。结果51例胆囊癌患者外周血CTCs阳性率为43．1％（22／51），CTCs与肝脏侵犯、Nevin分期具有显著的相关性（P〈0．05）。随访分析显示胆囊癌外周血CTCs阳性患者1年、2年总体生存率为70．7％、35．3％，外周血CTCs阴性1年、2年总体生存率为92．0％、56．1％，1年总体生存率差异无统计学意义（P〉0．05），2年总体生存率差异有统计学意义（χ^2=4．144，P〈0．05）。结论采用新型纳米微流控芯片能够有效检测胆囊癌外周血CTCs，其与胆囊癌的Nevin分期、肝脏侵犯密切相关，可作为判断胆囊癌患者预后的一个重要指标。Objective To establish a novel method using a nano microfluidic chip to detect circulating tumor cells （CTCs） in peripheral blood in gallbladder carcinoma, and to study the relationship between CTCs with clinicopathology and prognosis in these patients. Methods The peripheral blood samples of 51 patients with gallbladder carcinoma were collected from June 2014 to January 2017. The CTCs from peripheral blood samples were detected by a novel nano microfluidic chip. This study aimed to study the correlation between CTCs with the clinical and pathological features. The significance of CTCs on prognosis in patients with gallbladder carcinoma was also analyzed. Results The positive rate of CTCs in the peripheral blood of gallbladder carcinoma patients was 43.1% （22/51 ）. There were significant correlations between CTCs with liver metastasis （P 〈 0.05 ） and Nevin staging （P 〈 0.05 ）. The 1- and 2-year overall survival （OS） in patients with CTCs were 70.7% and 35.3%, and the 1- and 2-year OS in patients without CTCs were 92.0% and 56.1%. There was a significant difference in the 2-year OS （P 〈 0.05） but no significant difference in the 1-year OS between the 2 groups of patients （P 〉 0. 05）. Conclusions Peripheral blood CTCs in patients with gallbladder carcinoma were efficaciously detected by a novel nano microfluidic chip. Peripheral blood CTCs was closely related to the Nevin staging and liver metastasis. CTCs could serve as a potential prognostic indicator in patients with gallbladder carcinoma.

关 键 词：胆囊癌 微流控芯片 纳米 循环肿瘤细胞 预后 

分 类 号：R735.8[医药卫生—肿瘤]