玉米核基因的图位克隆方法  

Nuclear Gene Isolation by Map-based Cloning in Maize

作　　者：黄世全[1,2,3] 王棚涛[1,2,3] 郭思义 宋纯鹏[1,2,3] HUANG Shiquan;WANG Pengtao;GUO Siyi;SONG Chunpeng(Institute of Plant Stress Biology;State Key Laboratory of Cotton Biology;College of Life Science, Henan University, Henan Kaifeng 475004, China)

机构地区：[1]河南大学河南省植物逆境生物学重点实验室,河南开封475004 [2]河南大学棉花生物学国家重点实验室,河南开封475004 [3]河南大学生命科学学院,河南开封475004

出　　处：《河南大学学报（自然科学版）》2018年第3期318-327,共10页Journal of Henan University:Natural Science

基　　金：NSFC-河南联合基金重点资助项目(U1604233)

摘　　要：玉米是主要的粮食和饲料作物,经济地位十分重要,玉米杂种优势的利用是农作物杂种优势利用最成功的例证之一.图位克隆是鉴定突变体突变基因的重要方法之一.随着不同植物基因组数据的释放和新的技术出现,图位克隆将得到更为广泛的应用.本文概述了图位克隆的基本原理及其在玉米基因克隆中的常用操作方法,包括遗传特征的分析、作图群体的构建、多态性分子标记的筛选、目的基因的粗定位和精细定位、候选基因的评估和验证等,最后讨论图位克隆在玉米核基因克隆的应用中可能出现的问题和解决措施.本文所描述的应用于玉米突变体的图位克隆的常规策略在植物里是相通的,也适用于其他植物.Maize（Zea mays）is one of the most important food and crops with important economic significance,and the utilization of maize heterosis is one of the most successful examples of crops.Map-based cloning is a forward genetic approach to identify the molecular lesions causing mutant phenotypes.With the release of genomic data from different plants and the emergence of new technologies,map-based cloning will be more widely used.Here we outlined the basic principles of map-based cloning and its protocol in maize gene isolation,including genetic analysis,mapping population construction,polymorphic molecular marker screening,rough and fine mapping of target gene,candidate gene evaluation and validation,and finally discussed the possible problems and solutions of map-based cloning in the applications of maize gene cloning.The general mapping strategy applied to maize mutants described here is common to plants and should be used in other plants.

关 键 词：玉米 核基因 图位克隆 SSR BSA 

分 类 号：S513[农业科学—作物学]