一种清瘟解毒方在小鼠体内的抗甲型流感FM1病毒株活性  

Antiviral effect of a Qingwen Jiedu formula on the influenza A virus FM1 strain in mice

作　　者：徐琳琳 孙国倩 郭静 王晓龙 阎春生[1] 杨静[2] XU Lin-lin;SUN Guo-qian;GUO Jing;WANG Xiao-long;YAN Chun-sheng;YANG Jing(College of Public Health, Lanzhou University, Lanzhou 730000, China;Biotechnology Laboratory, Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China)

机构地区：[1]兰州大学公共卫生学院,兰州730000 [2]军事科学院军事医学研究院辐射医学研究所,北京100850

出　　处：《国际药学研究杂志》2018年第1期32-36,共5页Journal of International Pharmaceutical Research

基　　金：国家自然科学基金资助项目(31270197;81473184);国家重大专项课题资助项目(2016ZX09J16104-001-003;2015ZX09J15105)

摘　　要：目的研究一种清瘟解毒方对体内甲型流感病毒的影响,观察其是否具有抗流感病毒作用。方法将BALB/c小鼠随机分为正常对照组(给等体积生理盐水)、模型对照组(给等体积生理盐水)、阳性药对照组[给达菲10 mg/(kg·d)]、清瘟解毒方5、10、20 g/(kg·d)剂量组。正常对照组小鼠用PBS 20μl滴鼻,其余各组小鼠均采用等体积流感病毒稀释液滴鼻造模。感染病毒2 h后灌胃给药,连续给药6 d。以小鼠死亡率、平均存活时间、死亡保护率为指标,观察该方不同剂量对流感病毒感染小鼠的保护作用;综合肺指数、肺组织病毒载量、炎症因子(TNF-α、IFN-γ和IL-6)水平等指标,整体考察该方抗流感病毒的效果。结果与模型对照组相比,该方5、10、20 g/(kg·d)3个剂量组小鼠平均存活天数明显增加,死亡保护率明显提高,各剂量组死亡率明显降低(P<0.01);该方10和20 g/(kg·d)组小鼠在造模后第3天和第6天的肺指数、肺病毒RNA含量明显降低(P<0.01),血清炎性因子IL-6和TNF-α含量也显著降低(P<0.01),而血中IFN-γ的表达水平则均较模型对照组明显升高(P<0.01),其中尤以10 g/(kg·d)剂量组更为显著。结论清瘟解毒方在小鼠体内具有一定的抗甲型流感病毒作用,有望开发成新的抗流感病毒复方。Objective To investigate the antiviral effect of a Qingwen Jiedu formula（QJF）on the influenza A virus FM1 strain using the virus-infected mouse model. Method BALB/c mice were randomly divided into the normal control group（to administer the same volume of saline）,model control group（to administer the same volume of saline）,positive drug control group[to administer the 10 mg/（kg·d）of oseltamivir phosphate],and the 5,10 and 20 g/（kg·d）QJF-test groups,respectively. The mice in the normal control group were intranasally instilled with PBS 20 μl,while the others were infected with the equal volume of diluted influenza virus solutions via the same intranasal way. After two hours of the virus infection,the drugs or saline solutions were orally administered to the mice once a day for successive six days. The mortality,average of survival time and protection rate on the animal death were determined to evaluate the protective effect of QJF on the virus-infected mice. Furthermore,the lung index,lung tissue viral load and serum levels of the inflammatory factors,TNF-α,IFN-γ,IL-6,were also detected as the related indicators for the effect of QJF. Results Compared with the model control group,the mortality of the mice was significantly decreased together with the significant improvement of the average survival time and protection rate in the 5,10 and 20 g/（kg·d）QJF groups（P〈0.01）. Further in the10 and20 g/（kg·d）QJF groups,the lung index and lung tissue viral load decreased significantly both at the third day and sixth day of the virus infection（P〈0.01）. Meanwhile,the serum IL-6 and TNF-α levels were also significantly decreased（P〈0.01）,while the serum IFN-γ levels increased significantly in the 10 and 20 g/（kg·d）QJF groups（P〈0.01）than in the model control group,especially with a more significant increase in the 10 g/（kg·d）QJF group. Conclusion QJF showed an antiviral effect on the influenza A virus FM1 strain in the in vivo test in mice,which was thus expect

关 键 词：清瘟解毒方 流感病毒 肿瘤坏死因子Α 白细胞介素6 Γ-干扰素 

分 类 号：R978.7[医药卫生—药品]