白藜芦醇通过P38 MAPK通路参与对小肠缺血再灌注继发肺损伤的保护作用  

P38Mapk signaling pathway participates protective effect of resveratrol in lung injury induced by intestinal ischemia/reperfusion in rats

作　　者：王晓光[1] 李戈辉[1] 齐晓非[1] WANG Xiaoguang;LI Gehui;QI Xiaofei(Department of Anesthesiology Shenzhen Maternity and Child Healthcare Hospital, Shenzhen 518028, China)

机构地区：[1]深圳市妇幼保健院麻醉科,518028

出　　处：《心肺血管病杂志》2018年第5期478-481,共4页Journal of Cardiovascular and Pulmonary Diseases

基　　金：2016年深圳市科创委基金项目(JCYJ20160427192121979)

摘　　要：目的:探讨白藜芦醇是否通过p38丝裂原激活蛋白激酶(p38 MAPK)信号通路参与对肠缺血再灌注继发肺损伤的保护。方法:健康成年,SD大鼠,36只,随机分为正常对照组(C组,n=12)、缺血再灌注组(IR组,n=12),缺血再灌注+白藜芦醇预处理组(IRR组,n=12)。通过肠系膜动脉夹闭缺血45min后再开放再灌注4h复制大鼠小肠缺血再灌注继发肺损伤模型。计算肺湿干重比值(W/D),HE染色观察肺组织病理学变化,ELASIA法检测肺组织白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)表达,检测肺组织中磷酸化p38 MAPK(p-p38 MAPK)表达量。结果:病理切片可见IR组肺湿干比增加,组织染色可见肺泡上皮细胞肿胀,肺泡壁增宽,肺间质和肺泡腔水肿明显,肺泡内炎症细胞、红细胞和蛋白渗出明显增多。炎性因子(TNF-α,IL-6)表达增加,p38MAPK表达增加。用白藜芦醇预处理后,W/D比IR组减少,肺损伤程度较IR组减轻,p38表达较IR组减少。结论:白藜芦醇可能通过p38MAPK通路参与保护肠缺血再灌注造成的肺损伤。Objective： To explore the protective effect of resveratrol on acute lung injury induced by intestinal ischemia/reperfusion in rats. Methods： 36 adult male SD rats were randomized to three groups： control group was sham-operated(sham,n = 12）;（2） ischemia/reperfusion group(IR,n = 12）（3）I-R ＋ resveratrol group(IRR,n = 12）. The model of secondary lung injury in rats was established by clamping the mesenteric artery to clamp the ischemic 45 min and reopening and reperfusion after 4 h. The pathological changes of lung tissue were observed by HE staining. The expression of interleukin 6(IL-6） and tumor necrosis factor alpha(TNF-α） in lung tissue was detected,and the ratio of lung wet dry weight(W/D） was calculated. The expression of phosphorylated p38 MAPK(P-p38 MAPK） in lung tissue was detected. Results： Lung wet/dry weight ratio,the pathological lung injury,and the expression level of p38 MAPK in lung tissue in IR group were significantly increased,while the above indexes in group IRR were decreased compared to IR group(P〈0. 05）.Conclusion： Resveratrol have protective effects on the lungs against intestinal I-R-induced injury,and p38 MAPK signaling pathway participates protective effect of resveratrol.

关 键 词：白藜芦醇 P38-MAPK 缺血再灌注肺损伤 

分 类 号：R54[医药卫生—心血管疾病]