抗癌膏穴位贴敷对Lewis肺癌模型小鼠癌细胞周期移行的影响  

Effects of Acupoint Application of Anti-Cancer Plaster on Cancer Cell Cycles in Lewis Lung Cancer Model Mice

作　　者：孙六合[1] 杨庆有[1] 

机构地区：[1]河南中医学院针推系,郑州450008

出　　处：《中国针灸》2002年第9期625-627,共3页Chinese Acupuncture & Moxibustion

摘　　要：目的:探索抗癌膏穴位贴敷治疗癌症的作用机制。方法:用C57BL/6近交系小鼠,瘤细胞悬液皮下接种法造模,造模5日后用抗癌膏贴敷接种部位及“肺俞”穴,隔日治疗1次,治疗7次后,用流式细胞技术进行癌细胞周期分析。结果:抗癌膏使G1期细胞比例显著增加（P<0.05）,S期细胞比例显著减少（P<0.01）,细胞增殖指数显著下降（P<0.05）,癌细胞周期移行在G1期到S期之间发生阻滞。结论:抗癌膏通过抑制癌细胞增殖起到抗癌作用。Purpose To explore the mechanism of acupoint application of Anti-Cancer Plaster for treatment of cancer. Methods Lewis lung cancer model mice were established by subcutaneous vaccination of suspension of tumor cells in C57 BL/6 inbreeding mice. Five day later, Anti-Cancer Plaster was applied at the vaccinated position and point 'Feishu' (BL 13), once every other day, for 7 sessions of treatment, and then flow cytometry was adopted to analyse the cancer cell cycles. Results After treatment, the cancer cell cycle was blocked while shifting from GI phase to S phase; the proportion of cells in G1 phase increased significantly ( P < 0.05 ); the proportion of cells in Sphase decreased significantly (P < 0.01); the index of cell proliferation decreased significantly (P < 0.05). Conclusion Anti-Cancer Plaster plays an action of anti-cancer through depressing proliferation of cancer cells.

关 键 词：抗癌膏 穴位贴敷 肺肿瘤 穴位疗法 细胞周期 药理学 

分 类 号：R273.42[医药卫生—中西医结合] R285[医药卫生—中医肿瘤科]