螺内酯对糖尿病肾病大鼠肾脏GRKs与GPR17表达的影响与意义  

Influence and significance of spirolactone on kidney GRKs and GPR17 expressions of diabetic nephropathy rats

作　　者：陆杨[1] 羊红玉[2] 吴昂[3] LU Yang;YANG Hong-yu;WU Ang(Pharmacy, Hangzhou Cancer Hospital, Hangzhou, Zhejiang 310002, China)

机构地区：[1]杭州市肿瘤医院药剂科,浙江杭州310002 [2]浙江大学医学院附属第一医院药剂科,浙江杭州310003 [3]杭州市肿瘤医院内二科,浙江杭州310002

出　　处：《中国卫生检验杂志》2018年第10期1180-1183,1211,共5页Chinese Journal of Health Laboratory Technology

基　　金：2015年省医药卫生一般研究计划(A类)(2015KYA095)

摘　　要：目的探讨螺内酯对糖尿病肾病大鼠肾脏G蛋白偶联受体激酶(GRKs)与G蛋白偶联受体17(GPR17)表达的影响。方法将60只SPF级SD大鼠随机分为3组,各组20只。对照组正常饲养,其余2组制备糖尿病肾病模型,螺内酯干预组每日以100 mg/kg的螺内酯灌胃。取24 h尿液和腹腔静脉血测定血脂、血糖与肾功能;取肾脏,测定肾脏指数(RI)与肾小球硬化指数(GI),并采用WB实验测定GRK2、GRK3、GRK4和GRK6和GPR17表达。结果与对照组相比,模型组和螺内酯干预组RI和GI升高;与模型组相比,螺内酯干预组RI和GI降低,差异有统计学意义(P<0.05)。与对照组相比,模型组的24 h尿白蛋白、血尿氮素(BUN)、血肌酐(Scr)、血糖、总胆固醇(TC)及甘油三酯(TG)水平升高,与模型组相比,螺内酯干预组24 h蛋白尿、BUN、Scr水平均降低,差异有统计学意义(P<0.05)。与对照组相比,模型组GRK2、GRK3、GRK4表达升高,GRK6和GPR17表达降低;与模型组相比,螺内酯干预组GRK2、GRK3、GRK4表达降低,GRK6和GPR17表达升高,差异有统计学意义(P<0.05)。结论螺内酯能够干预糖尿病肾病大鼠GRKs与GPR17表达,其具体机制尚需探索。Objective To explore the influence of spirolactone on kidney GRKs and GPR17 expressions of diabetic nephropathy rats. Methods Totally 60 SD rats were randomly divided into 3 groups,with 20 cases in each group. The rats of the control group were raised normally,while the other two groups were prepared as diabetic nephropathy models,at the same time,those of the spirolactone group were given 100 mg/kg spirolactone every day by gavage. The venous blood and urine within 24 h were collected to detect blood lipid,blood glucose and renal function. Renal index（ RI） and glomerulosclerosis index（ GI） were detected,and the protein expressions of GRK2,GRK3,GRK4,GRK6 and GPR17 were also detected by WB assay.Results Compared with the controls,the RI and GI levels of the model group and spirolactone group increased; and compared with the model group,the RI and GI levels of the spirolactone group decreased,and the difference between the two groups had statistical significance（ P〈0. 05）. Compared with the controls,the levels of urinary albumen,blood nitrogen（ BUN）,serum creatinine（ Scr）,blood glucose,total cholesterol（ TC） and triglyceride（ TG） of the model group increased,and compared with the model group,the levels of urinary albumen,BUN and Scr of the spirolactone group decreased,and the differences had statistical significance（ P〈0. 05）. Compared with the controls,the levels of GRK2 and GRK3 protein in the model group increased,while the levels of GRK4,GRK6 and GPR17 decreased,and compared with the model group,the levels of GRK2 and GRK3 in the spirolactone group decreased,while the levels of GRK4,GRK6 and GPR17 increased,and the difference had statistical significance（ P〈0. 05）. Conclusion Spirolactone has an effect on GRKs and GPR17 expressions of diabetic nephropathy rats,but its specific mechanism is still need to be explore.

关 键 词：螺内酯 糖尿病肾病 G蛋白偶联受体激酶 G蛋白偶联受体 

分 类 号：R587.2[医药卫生—内分泌]