传染性单核细胞增多症儿童外周血T细胞亚群的变化及意义  

Changes and significance of T cell subsets in peripheral blood of children with infectious mononucleosis syndrome

作　　者：邢超[1] 邵美娟[1] 杨建环[2] 宫剑[1] XING Chao;SHAO Mei-juan;YANG Jian-huan;GONG Jian(Department of Laboratory Medicine, the Second Affiliated Hospital of Wenzhou Medical University, Yuying Children＇s Hospital, Wenzhou, Zhejiang 325027, Chin)

机构地区：[1]温州医科大学附属第二医院育英儿童医院检验科,浙江温州325027 [2]温州医科大学附属第二医院育英儿童医院儿科,浙江温州325027

出　　处：《中国卫生检验杂志》2018年第10期1206-1208,共3页Chinese Journal of Health Laboratory Technology

基　　金：温州市公益性科技计划项目(Y20160507);浙江省自然科学基金(LH17H200002)

摘　　要：目的分析传染性单核细胞增多症(IM)患儿外周血T细胞亚群的变化及意义,为IM患者的临床诊断和治疗提供理论依据。方法收集2016年1月-2017年1月在温州医科大学育英儿童医院就诊的IM患儿65例为IM(IM组),以同期在本院儿童保健科体检的儿童30例作为对照组。采用流式细胞术检测患者外周血CD3+CD4+T淋巴细胞、CD3+CD8+T淋巴细胞、Treg细胞、Th17细胞频数变化。结果 IM组CD3+CD8+细胞频数、Th17细胞明显高于对照组,CD3+CD4+、CD4/CD8、Treg/Th17明显低于对照组,差异均有统计学意义(P<0.05)。IM患者急性期EBV-DNA病毒载量与CD3+CD8+细胞、Th17细胞频数呈正相关(r值分别为0.722、0.797,P<0.01);与CD3+CD4+频数、Treg细胞、CD4/CD8及Treg/Th17比值呈负相关(r值分别为-0.421、-0.357、-0.469、-0.422,P<0.05)。结论 IM患者存在细胞免疫功能紊乱,其免疫功能紊乱程度与EB病毒感染量有关,调节性T细胞减少和Th17细胞增多可能参与IM发病机制。Objective To analyze the changes and significance of T lymphocyte subsets in peripheral blood of children with infectious mononucleosis（ IM）,so as to provide theoretical basis for the clinical diagnosis and treatment of IM patients. Methods Sixty-five children with IM were enrolled in IM group from January 20 to January 2017 in Yuying Children＇s Hospital,Wenzhou Medical University,and 30 children were enrolled as healthy control group（ HC group） from the Child Health Care Unit during the same period. The levels of CD3＋CD4＋T lymphocytes,CD3＋CD8＋T lymphocytes,Treg cells and Th17 cells were by flow cytometry.Results The levels of CD3＋CD4＋,CD4/CD8 and Treg/Th17 were significantly lower in the IM group than that in the control group,with the differences statistically significant（ P〈0. 05）. The loading of EBV-DNA virus in IM patients was positively correlated with the frequency of CD3＋CD8＋cells and Th17 cells（ r = 0. 722,r = 0. 797,P〈0. 01）; and that were negatively correlated with CD3＋CD4＋frequency,Treg cells,CD4/CD8 and Treg/Th17 ratio（ r =-0. 421,r =-0. 357,r =-0. 469,r =-0. 422,P〈0. 05）. Conclusion There are cellular immunity dysfunction in IM patients. The degree of immune dysfunction is related to the amount of EB virus infection. The decrease of regulatory T cells and the increase of Th17 cells may be involved in the pathogenesis of IM.

关 键 词：传染性单核细胞增多症 淋巴亚群 调节T细胞 TH17细胞 细胞因子 

分 类 号：R725.1[医药卫生—儿科]