高效液相色谱法测定决明退障口服液中沙苑子苷、芍药苷的含量  

Content Determination of Complanatuside and Paeoniflorin in Juemingtuizhang Oral Liquid by HPLC

作　　者：陈玲玲 金璇 赵颖[1] CHEN Lingling;JIN Xuan;ZHAO Ying(Dept. of Pharmacy, Nanjing Hospital of Traditional Chinese Medicine, Jiangsu Nanjing 210001, China)

机构地区：[1]南京市中医院药学部,江苏南京210001

出　　处：《中国医院用药评价与分析》2018年第4期449-451,共3页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：江苏省南京市医学科技发展项目资助(No.YKK14141)

摘　　要：目的:建立高效液相色谱法测定决明退障口服液中沙苑子苷、芍药苷的含量。方法:(1)测定沙苑子苷的含量。色谱柱为Aglient ZORBAX Eclipse Plus C_(18)柱(2.1 mm×50 mm,1.8μm);流动相为乙腈-0.1%磷酸溶液(V∶V=21∶79);检测波长为266 nm。(2)测定芍药苷的含量。色谱柱为Diamonsil C_(18)(2)柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸溶液(V∶V=14∶86);检测波长为230 nm。结果:沙苑子苷标准曲线回归方程为Y=21 338 X-29.63,r=0.999 6(n=6),进样量在1~32μg范围内线性关系良好,沙苑子苷的转移率为80.31%;芍药苷标准曲线回归方程Y=12 467 X-41.99,r=0.999 9(n=6),进样量在1~48μg范围内线性关系良好,芍药苷的转移率为64.69%。结论:本方法能够准确测定决明退障口服液中沙苑子苷、芍药苷的含量,为决明退障口服液的质量控制提供依据。OBJECTIVE： To establish the method for content determination of complanatuside and paeoniflorin in juemingtuizhang oral liquid by HPLC. METHODS ： （ 1 ） To determine the content of complanatuside. The column was Aglient ZORBAX Eclipse Plus C18 （2. 1 mm×50 mm, 1.8 μm）, the mobile phase was acetonitrile-0. 1% phosphoric acid solution （V： V = 21：79）, and the detective wavelength was 266 nm. （2） To determine the content of paeoniflorin. The column was Diamonsil C1s（2） （250 mm×4.6 mm,5 μm）, the mobile phase was acetonitrile-0. 1% phosphoric acid solution （V： V= 14：86）, and the detective wavelength was 230 nm. RESULTS： The standard curve regression equation of complanatuside was Y = 21 338 X - 29.63, r = 0. 999 6 （ n = 6）, the sample size was well linear in the range of 1-32 μg, and the transfer rate was 80. 31%. In addition, the standard curve regression equation of complanatuside was Y = 12 467 X - 41.99, r = 0. 999 9 （ n = 6 ） , the sample size was well linear in the range of 1-48 μg, and the transfer rate was 64.69%. CONCLUSIONS： This method can be used for accurate content determination of complanatuside and paeoniflorin in juemingtuizhang oral liquid, and provide basis for quality control of juemingtuizhang oral liquid.

关 键 词：高效液相色谱法 决明退障口服液 沙苑子苷 芍药苷 

分 类 号：R927.2[医药卫生—药学]