半枝莲水提液和去纳米颗粒水提液在体内外抗肝肿瘤作用的对比研究  

Comparative Study on Effects of Water-extraction of Scutellaria Barbata(SEB) and Water-extraction of Scutellaria Barbata with Nanoparticles Removed(SEB-RN) on Anti-liver Tumor in Vitro and in Vivo

作　　者：黄新伟[1] 郑建华[1] 陈文滨[1] 程金生 HUANG Xinwei1 ZHENG Jianhua1 CHEN Wenbin1 CHENG Jinsheng2(1. Dept. of Pharmacy Huizhou the First People＇s Hospital, Guangdong Huizhou 516003, China; 2. Hakka Health Care Research, Medical College of Jiaying University, Guangdong Meizhou 514031, Chin)

机构地区：[1]惠州市第一人民医院药学部,广东惠州516003 [2]嘉应学院医学院客家养生保健研究所,广东梅州514031

出　　处：《中国医院用药评价与分析》2018年第5期654-656,659,共4页Evaluation and Analysis of Drug-use in Hospitals of China

摘　　要：目的:探讨半枝莲水提液(SEB)和去纳米颗粒水提液(SEB-RN)体内外的抗肝肿瘤作用。方法:采用体外抑瘤实验培养人肝癌细胞SMMC-772,通过噻唑蓝(MTT)法检测其体外肿瘤抑制率;体内实验采用肝癌H22移植瘤法,以给药前后体质量、肿瘤抑制率、脾脏指数和胸腺指数为指标,对比SEB和SEB-RN的肿瘤抑制作用。结果:体外试验结果表明,环磷酰胺(CTX)组、SEB组和SEB-RN组均对SMMC-7721细胞的增殖有显著抑制作用;与SEB-RN组比较,SEB组对SMMC-7721细胞的增殖抑制率明显更高。体内试验结果表明,与模型组比较,CTX组、SEB组和SEB-RN组均能显著抑制实体瘤生长,与SEB-RN组比较,SEB组肿瘤抑制率明显更高;与模型组比较,CTX组胸腺、脾脏指数明显更低,SEB组、SEB-RN组明显更高,差异均有极显著统计学意义(P<0.01);SEB组胸腺、脾脏指数明显高于SEB-RN组,差异有统计学意义(P<0.05)。结论:SEB和SEB-RN均有一定的抗肿瘤和增强免疫功能的作用,但SEB优于SEB-RN。OBJECTIVE： To probe into the effects of water-extraction of scutellaria barbata（ SEB） and waterextraction of scutellaria barbata with nanoparticles removed（ SEB-RN） on Anti-liver tumor in vitro and in vivo.METHODS： In vitro tumor inhibition test was performed to culture SMMC-772,and MTT method was used to determine the proliferation of SMMC-772 in vitro. Liver cancer H22 transplantation tumor method was adopted for in vivo experiment; body mass,tumor inhibitory rate,spleen index and thymus index before and after administration were considered as indicators,the inhibitory effect on tumor was compared between SEB and SEB-RN. RESULTS： In vitro test show that： the CTX group,SEB group and SEB-RN group had significant inhibitory effects on the proliferation of SMMC-772; and the SEB group was higher than the SEB-RN group. In vivo test show that： compared with the model group,CTX group,SEB group and SEB-RN group could significantly inhibit the growth of solid tumor,and SEB group was significantly higher than the SEB-RN group. Compared with the model group,the thymus and spleen index of CTX were significantly lower,yet the data in SEB group and SEB-RN group were significantly higher,the difference was statistically significant（ P〈0. 01）; the thymus and spleen index of SEB group were significantly higher than those of SEB-RN group,with statistically significant difference（ P〈0. 05）. CONCLUSIONS： SEB and SEB-RN both have certain anti-tumor effects and improvement effects on immune function,yet the SEB is superior to SEB-RN.

关 键 词：半枝莲 纳米颗粒 水提液 抗肝肿瘤 

分 类 号：R932[医药卫生—生药学]