猪流行性腹泻病毒感染Vero细胞的细胞病理学研究  

Cytopathology study on porcine epidemic diarrhea virus(PEDV)infected vero cells

作　　者：闫清源 周书亭 谢洋洋[1] 丁思嘉[1] 郑煜明 罗依然[1] 华修国[1] 杨志彪[1] YAN Qing-yuan;ZHOU Shu-ting;XIE Yang-yang;DING Si-jia;ZHENG Yu-ming;LUO gi-ran;HUA Xiu-guo;YANG Zhi-biao(Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China;College of Fisheries and Life Science,Shanghai Ocean University, Shanghai 201306,China)

机构地区：[1]上海交通大学农业与生物学院上海市兽医生物技术重点实验室,上海200240 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《上海交通大学学报（农业科学版）》2018年第2期39-42,共4页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家自然科学基金(31472211);国家重点研发计划(2016YFD0500100);上海市自然基金(14ZR1421400)

摘　　要：对感染猪流行性腹泻病毒(PEDV)的Vero细胞进行透射电镜观察发现:在病毒感染初期(6h),尽管细胞结构完整,部分细胞线粒体、粗面内质网扩张,胞浆出现空泡;随着感染时间的增长(12h),病毒粒子逐渐增多,细胞质内出现较多空泡状结构,病毒粒子先后出现在胞浆及细胞核内部;感染24h后,病毒粒子数目显著增多,空泡化明显,出现细胞核分解,细胞融合更加明显;感染最后阶段(48h),空泡化严重,核质固缩,细胞间大量融合,病毒粒子出芽排出。以上结果,为揭示猪流行性腹泻病毒的感染与致病机制积累了数据。Transmission electron microscope （TEM） was employed to investigate the pathological changes in Vero cells infected by porcine epidemic diarrhea virus （PEDV）.It is observed that at the incipient phase of the infection （6 h）,though with intact cellular structure,part of mitochondria and rough endoplasmic retiulums expanded,cytoplasm vacuolization emerged.With the infection lasting （12 h）,the virus particles gradually increased,more vacuolar structures appeared in cytoplasm,and virus particles successively appeared in cytoplasm and nuclei.As infection lasting 24 hours,with an observably increasing viron amount,the mitochondria vacuolization and cell fusion became more prominent,even nucleus lysis was observed.At the final phase of infection （48 h）,with severe vacuolization,nuclear condensation and cell infusion,the neonatal PEDV virons were budding from the infected Vero cells.All the results above laid a data foundation for unveiling the infection and pathogenic mechanism of PEDV.

关 键 词：猪流行性腹泻病毒 VERO细胞 细胞病理学 

分 类 号：S858.28[农业科学—临床兽医学] S852.659.2[农业科学—兽医学]