IL-4基因敲除小鼠脑外伤后脑水肿程度及血清HMGB1和S100B水平变化  

Worsened brain edma and the level of serum HMGB1 and S100B of IL-4 knockout mice after trau- matic brain injury

作　　者：叶樱泽 熊晓星[2] 古丽娟[1] Ye Yingze ,Xiong Xiaoxing, Gu Lijuan(Central Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, China ; Department of Neurosurgery , Renmin Hospital of Wuhan University, Wuhan 430060, China)

机构地区：[1]武汉大学人民医院中心实验室,武汉430060 [2]武汉大学人民医院神经外科,武汉430060

出　　处：《中华行为医学与脑科学杂志》2018年第5期385-389,共5页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（81771283,81301019,81571147）

摘　　要：目的研究IL-4基因敲除小鼠脑外伤后脑水肿以及血清高迁移率组蛋白（high mobility group box1，HMGB1）和钙结合蛋白S100B的变化，以探讨IL-4对脑外伤脑组织的作用。方法雄性野生型和IL-4基因敲除BALB／cJ小鼠各20只，分别随机分为假手术组和颅脑损伤组，每组10只。采用自由落体硬膜外撞击法建立局灶重度脑外伤模型，比较脑水肿含量；Westernblot检测各组小鼠损伤周围脑组织水通道蛋白4（aquaporin-4，APQ4）及HMGB1的表达；酶联免疫吸附（ELISA）法检测脑外伤后血清中HMGB1及S100B的表达。结果（1）脑外伤模型IL-4基因敲除BALB／cJ小鼠损伤侧脑组织含水量明显高于野生型小鼠[wT组：（80．03±0．35）％；IL-4基因敲除组：（81．93±0．41）％；P〈0．05]。（2）Westernblot结果显示，颅脑外伤后IL-4基因敲除BALB／cJ小鼠较野生型小鼠脑组织AQP4和HMGB1表达显著增高。（3）ELISA法结果显示，IL-4基因敲除BALB／cJ小鼠较野生型小鼠脑外伤后血清中HMGB1及S100B的水平明显增高[HMGB1含量：wT组：（9．21±0．74）ng／ml；IL-4基因敲除组：（13．39±1．33）ng／ml，P〈0．05；S100B蛋白含量：wT组：（11．11±0．84）pg／ml；IL-4基因敲除组：（18．11±2．02）pg／ml，P〈0．05]。结论IL-4基因敲除小鼠脑外伤后组织含水量和水通道蛋白-4的表达升高，脑组织和血清中HMGB1的水平升高，血清内钙结合蛋白S100B的含量升高。Objective To investigate the changes of brain edma and expression of blood high mobil- ity group box 1 （HMGB1） and calcium binding protein S100B after traumatic brain injury （TBI） in IL-4 knockout （IL-4 KO） mice,and to explore the effects of IL-4 on traumatic brain injury. Methods Twenty male wild type （WT） or twenty male IL-4 KO BALB/cJ mice were randomly divided into WT sham TBI group,WT TBI group,IL-4 KO sham TBI group and IL-4 KO TBI group（n= 10 in each group）.The model of traumatic brain injury was established by the free falling body epidural impact method, then the brain water content was measured.The expression of aquaporin-4 （APQ4） and HMGB1 in injured brain of each group was detected by Western blot, and the concentration of HMGB1 and S100B in serum was detected by ELISA assay. Results （1） The brain water content of injured lateral brain of BALB/cJ mice with IL-4 gene knockout was significantly higher than that of wild type mice with brain injury model （WT group： （80.03±0,35） % ;IL-4 KO group： （81.93±0,41）%; P〈0.05）. （2） The Western blot showed that the expression of AQP4 and HMGB1 in brain tissue of BALB/cJ mice with IL-4 gene knockout was significantly higher than those in wild type mice after traumatic brain injury. （ 3 ） The results of ELISA showed that the levels of HMGB1 and S100B in the serum of IL-4 knockout BALB/cJ mice were significantly higher than those of wild type mice （ HMGB1 ：WT group： （9.21±0.74） ng/ml;IL-4 gene knock-out group： （ 13.39±1.33）ng/ml,P〈 0.05;S100B protein：WT group： （11.11±0.84） pg/ml;IL-4 KO group： （18.11±2.02） pg/ml , P〈 0. 05 ）. Conclusion The brain tissue water content and the expression of APQ4 are increased in IL-4 KO TBI mice.The expression of HMGB1 in brain issue and serum and S100B in serum are also up-regulated.

关 键 词：颅脑外伤 白细胞介素-4 脑水肿 高迁移率组蛋白1 钙结合蛋白SIOOB 小鼠 

分 类 号：R651.15[医药卫生—外科学]