细粒棘球蚴感染者中Tim-3^+CD4^+CD25^+Treg细胞及相关因子的表达  

The expression of Tim-3^+CD4^+CD25^+Treg cells and the related factors in human Echinococcus granululosus infection

作　　者：赵慧[1] 王亮[1] 张峰波[1] 朱玥洁[2] 庞楠楠[3] 安梦婷 李斌 马秀敏[1] 丁剑冰[1,4] ZHAO Hui;WANG Liang;ZHANG Fengbo;ZHU Yuejie;PANG Nannan;AN Mengting;LI Bin;MA Xiumin;DING Jianbing(Center of Medical Laboratory, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China;Reproductive Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China;Blood Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China;Department of Immunology, College of Preclinical Medicine, Xinjiang Medical University, Urumqi 830011, China)

机构地区：[1]新疆医科大学第一附属医院医学检验中心,乌鲁木齐830011 [2]新疆医科大学第一附属医院生殖助孕中心,乌鲁木齐830011 [3]新疆医科大学第一附属医院血液中心,乌鲁木齐830011 [4]新疆医科大学基础学院免疫学教研室,乌鲁木齐830011

出　　处：《免疫学杂志》2018年第6期513-518,共6页Immunological Journal

基　　金：新疆维吾尔自治区自然科学基金(2016D01C325)

摘　　要：目的通过检测细粒棘球蚴感染者外周血中Tim-3^+CD4^+CD25^+Treg细胞及相关因子的表达水平,探讨细粒棘球蚴感染者中Tim-3与CD4+CD25+Treg细胞的关系及Tim-3+CD4+CD25+Treg细胞对该疾病持续性发展的作用。方法选取30例细粒棘球蚴感染者和30例健康对照人群,用流式细胞术检测感染组和对照组外周血中Tim-3+CD4+CD25+Treg细胞的比例变化;实时荧光定量PCR法分别检测感染组和对照组外周血单个核细胞中Tim-3和Foxp3 mRNA的表达;ELISA法检测感染组和对照组血清中IL-10和TGF-β的水平;Pearson相关法分析感染者外周血中Tim-3 mRNA的表达与Foxp3 mRNA的相关性以及Tim-3+CD4+CD25+Treg细胞与IL-10和TGF-β的相关性。结果与对照组相比,细粒棘球蚴感染组外周血中Tim-3^+CD4^+CD25+Treg细胞的比例均显著升高(P<0.001);外周血单个核细胞中Tim-3和Foxp3 mRNA水平显著升高(P<0.001);血清中IL-10(P<0.001)和TGF-β(P=0.046)水平显著升高;细粒棘球蚴患者外周血Tim-3 mRNA与Foxp3 mRNA表达水平呈正相关;Tim-3+CD4+CD25+Treg细胞与IL-10和TGF-β的水平均正相关。结论细粒棘球蚴感染者中Tim-3在CD4+CD25+Treg细胞上的过表达可能会促进CD4+CD25+Treg细胞的产生及其抑制功能的发挥,从而促使感染的持续性发展。This study was performed to explore the relationship between Tim-3 and CD4＋CD25＋Treg cells in Echinococcus granululosus infection and their roles in the development of the disease by detecting the expression of Tim-3＋CD4＋CD25＋Treg cells and related factors in Echinococcus granululosus infected patients and healthy controls.Flow cytometry was used to detect the ratio of Tim-3＋CD4＋CD25＋Treg cells in peripheral blood of 30 patients of Echinococcus granululosus and 30 healthy controls; Real-time fluorescent Quantitative PCR method was used for detecting the mRNA expression of Tim-3 and Foxp3 in the peripheral blood mononuclear cells; while ELISA method was used to detect the serum levels of IL-10 and TGF-β. Then Pearson method was applied to analyze the correlation between the expression of Tim-3 mRNA and Foxp3 mRNA and the correlation among the expression of Tim-3＋CD4＋CD25＋Treg cells, IL-10 and TGF-β. Data showed that compared with healthy control,Echinococcus granululosus infection group demonstrated significant higher ratio of Tim-3＋CD4＋CD25＋Treg cells in peripheral blood, significant higher mRNA levels of Tim-3 and Foxp3 in peripheral blood mononuclear cells, and significant higher levels of IL-10 and TGF-β in serum. Furthermore, the mRNA expression of Tim-3 and Foxp3 were positively correlated; the ratio of Tim-3＋CD4＋CD25＋Treg cells and the levels of TGF-β and IL-10 were also positively correlated. In conclusion,overexpression of Tim-3 on CD4＋CD25＋Treg cells surface may promote the production and function of CD4＋CD25＋Treg cells, which is benefit for sustainability development of human Echinococcus granululosus infection.

关 键 词：TIM-3 TREG 细粒棘球蚴感染 

分 类 号：R392.11[医药卫生—免疫学] R378.5[医药卫生—基础医学]