苦参醇A对缺氧/复氧损伤乳鼠心肌细胞的保护作用  

Protective Effects of Kushenol A on Cardiomyocytes of Neonatal Rats Suffering from Hypoxia/Reoxygenation Injury

作　　者：杨兴鑫 史婷婷[2] 董金材 王曦 李凤娇 顾雯[1] YANG Xingxin;SHI Tingting;DONG Jincai;WANG Xi;LI Fengjiao;GU Wen(College of Pharmaceutical Science, Yunnan University of Traditional Chinese Medicine, Kunming 650500 Yunnan, China;Department of Pharmaceutical Preparation, The Xixi Hospital of Hangzhou Affiliated to Zhejiang University of Traditional Chinese Medicine, Hangzhou 310023 Zhejiang, China)

机构地区：[1]云南中医学院中药学院,云南昆明650500 [2]浙江中医药大学附属杭州市西溪医院药剂科,浙江杭州310023

出　　处：《中药新药与临床药理》2018年第3期298-301,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金(81660596;81660684);云南省应用基础研究计划项目(2017FF117-013;2016FD050)

摘　　要：目的研究苦参醇A对缺氧/复氧损伤乳鼠心肌细胞的保护作用。方法培养乳鼠心肌细胞,建立心肌细胞缺氧/复氧损伤模型。实验分为5组:正常组,模型组(缺氧/复氧组),苦参醇A低、中、高剂量组,在缺氧/复氧开始时分别加入终浓度为30,60,120μg·m L^(-1)的苦参醇A。CCK-8法检测心肌细胞存活率,酶标仪检测细胞培养上清液中肌酸激酶(CK)、乳酸脱氢酶(LDH)漏出量及细胞内丙二醛(MDA)含量与超氧化物歧化酶(SOD)活力,流式细胞仪检测细胞凋亡率。结果苦参醇A可显著降低缺氧/复氧后CK与LDH漏出量(P<0.05,P<0.01),提高心肌细胞存活率(P<0.05,P<0.01);能显著降低MDA含量(P<0.05,P<0.01),提高SOD活性(P<0.05,P<0.01);并能显著抑制心肌细胞凋亡(P<0.05,P<0.01)。结论苦参醇A对培养心肌细胞的缺氧/复氧损伤具有保护作用,其机制可能与减少氧化应激产物MDA,增加抗氧化酶SOD活力及抑制心肌细胞凋亡有关。Objective To investigate the protective effects of kushenol A on cardiomyocytes of neonatal rats sufferingfrom hypoxia/reoxygenation injury. Methods Cardiomyocytes of neonatal rats were cultured in vitro,and then wereused to establish hypoxia/reoxygenation injury model of cardiomyocytes. Cultured cardiomyocytes were divided intofive groups： normal group, model group（hypoxia/reoxygenation group）and kushenol A groups（30, 60 and 120μg·m L-1）. At the beginning of hypoxia/reoxygenation,the kushenol A with final concentrations of 30,60 and 120μg·m L-1 were added into the culture medium separately. The viability of cardiomyocytes was determined using cellcounting kit-8 assay（CCK-8）. The leakage of creatine kinase（CK）and lactate dehydrogenase（LDH）in supernatant ofcardiomyocytes culture medium, as well as the malondialdehyde（MDA）content and superoxide dismutase（SOD）activity in cardiomyocytes were detected by microplate reader. Moreover,the apoptosis ratio was detected by flowcytometry. Results Kushenol A could remarkably reduce the CK and LDH leakage after hypoxia/reoxygenation,withthe increasing of cardiomyocyte viability（P 〈 0.05, P 〈 0.01）. Furthermore, it could significantly decrease theMDA content, increase the SOD activity and inhibit the apoptosis of cardiomyocytes（P 〈 0.05, P 〈 0.01）.Conclusion Kushenol A could protect the cultured cardiomyocytes suffering from hypoxia/reoxygenation injury. The action mechanism may be related to the reduction of oxidative stress product（MDA）,enhancement of antioxidantenzyme activity（SOD）,and inhibition of apoptosis of cardiomyocytes.

关 键 词：苦参醇A 心肌细胞 缺氧/复氧 氧化应激 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]