Catsperg1基因在大鼠睾丸发育中转录表达的分析  

Analysis of Catsperg1 expression in the development of rat testis

作　　者：韦龙生[1,2] 韩璐 杨铮[1] 王丽佳[1] 王羚鸿 刘陶迪 张信来[3] Wei Longsheng;Han Lu;Yang Zheng;Wang Lijia;Wang Linhong;Liu Taodi;Zhang Xinlai(1. Department of Psychosomatic Medicine, lnner Mongolia Medical University, Hohhot 010110, Inner Mongolia;Fenyang College Shanxi Medical University Shanxi, Fenyang;Department of General Surger24 the First Affiliated Hospital, Inner Mongolia Medical University)

机构地区：[1]内蒙古医科大学心身医学研究室,内蒙古呼和浩特010110 [2]山西医科大学汾阳学院 [3]内蒙古医科大学附属医院普外科

出　　处：《中国男科学杂志》2018年第2期16-19,共4页Chinese Journal of Andrology

基　　金：国家自然科学基金(项目批准号:81260111);内蒙古医科大学生殖生物学人才团队(项目编号NYTD-2015003)

摘　　要：目的研究分析Catsperg1基因在大鼠睾丸发育中的转录表达,探究该基因对精子所起的作用。方法在出生后2到65日龄21个时间点的雄性Wistar大鼠中,每个时间点取3只不同窝别的睾丸组织,用试剂盒提取总mRNA,并反转录成cDNA,随后采用Real-time PCR方法检测大鼠睾丸组织中Catsperg1基因mRNA的转录情况。结果Catsperg1基因mRNA表达在鼠出生后第20天后开始缓慢上升,在第50、55天达到最高峰,在第60、65天仍维持一定峰值。结论该基因在大鼠睾丸组织中晚期发育的表达量逐渐升高,可能参与精子成熟过程。Objective To study the transcriptional expression of Catspergl in the development of rat testes and its effect on sperm maturation. Methods Male Wistar rats at 21 time points of age （frome 2 to 65 days） were given, and their testis tissues at three different litter were collected at each time point. Total mRNA was extracted from rat testis tissues and reverse transcribed into eDNA, and then amplified for detection the transcription expression of Catspergl by real-time PCR. Results The mRNA expression of Catspergl began to increase slowly at the 20th day after birth, reached the peak at the 50th and 55th day, and remained at the peak at the 60th and 65th day. Conclusion The expression of Catspergl in rat testis gradually increased, which may be involved in the regulation of sperm maturation.

关 键 词：Catsperg1 基因表达 精子发生 大鼠 精子成熟 

分 类 号：R339.21[医药卫生—人体生理学]