氮硫配施对苗期菘蓝中营养物质及活性成分的影响  

Effects of nitrogen and sulfur combined application on nutritional components and active components of Isatis indigotica at seedling stage

作　　者：缪雨静 关佳莉 曾佳乐 徐菁 唐晓清[1] MIAO Yu-jing;GUAN Jia-li;ZENG Jia-le;XU Jing;TANG Xiao-qing(Institute of Chinese Medicinal Materials, Nanfing Agricultural University, Nanjing 210095, China)

机构地区：[1]南京农业大学中药材研究所

出　　处：《中国中药杂志》2018年第8期1571-1578,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(31171486)

摘　　要：为探讨氮硫配施对苗期菘蓝生长,营养状况及活性成分的影响,采用裂区试验设计,研究不同氮素水平（N：5,15,25mmol·L^-1）,硫素水平（S：0.00,1.25,2.50,5.00,7.50 mmol·L^-1）对盆栽砂培菘蓝生物量,营养物质,活性成分的影响。结果表明,氮硫2种元素配施对苗期菘蓝生长,营养物质及次生代谢物的积累具有显著效应。N2水平下菘蓝叶片和根干重处于最高水平,在相同的氮素浓度下,菘蓝叶片和根干重均随着硫素浓度的升高呈先上升后下降趋势,其中以S2浓度的硫素较有利于菘蓝生长及干物质积累。可溶性糖,可溶性蛋白,游离氨基酸含量分别以N1,N2,N3水平下最高,硫素对营养物质的影响基本随着硫素浓度的增加呈先上升后下降趋势,但不同氮素水平下各营养物质峰值存在差异。氮硫互作对次生代谢产物总黄酮,靛蓝,靛玉红,（R,S）-告依春含量影响规律不明显,分别以N1S3,N1S2,N3S0,N3S1最高。综合考虑,氮素浓度在15 mmol·L^-1,硫素浓度在2.50 mmol·L^-1时较适合菘蓝叶及根的生长,活性成分也处于相对较高水平。该研究可以为菘蓝的合理施肥及优质栽培提供一定的理论基础。Using split plot design,a pot experiment with sand culture was conducted to investigate the effects ofnitrogen and sulfur combined application on nutritional components and active component of Isatis indigotica at seedling stage under different N（ 5,15,25 mmol·L^-1） and S（ 0. 00,1. 25,2. 50,5. 00,7. 50 mmol·L^-1） levels. The results showed thatthe two elements had obvious effects and the leaf and root dry weights of I. indigotica seedlings increased greatly at N2 level. Under the same nitrogen concentration,the leaf and root dry weights increased firstly and decreased with the rising of sulfur concentrations in which S2 was conducive to the growth and biomass accumulation. Soluble sugar,soluble protein,soluble amino acids contents were the highest in N1,N2 and N3 treatments,respectively. The influence of sulfur concentrations on nutritional components was same as biomass,but the peak of different nutritional components was diversity in different nitrogen levels. The effects on secondary metabolites（ total flavones,indigo,indriubin,epigotrin contents） were not obvious significantly,in which these indexes by N1S3,N1S2,N3S0,N3S1 were the highest,respectively. In conclusion,the combination of nitrogen and sulfur of N2S2（ N 15 mmol·L^-1 and S 2. 5 mmol·L^-1） was beneficial to the growth and secondary metabolites accumulation of I. indigotica. These results could provide a theoretical basis for rational fertilization and cultivation of I. indigotica seedling.

关 键 词：菘蓝 氮硫配施 营养物质 靛蓝 靛玉红 (R S)-告依春 

分 类 号：S567.239[农业科学—中草药栽培]