糖尿病因素对腺苷后处理减轻大鼠心肌缺血再灌注损伤的影响  

Effect of diabetes mellitus on adenosine postconditioning-induced reduction of myocardial ischemia-reperfusion injury in rats

作　　者：陈超[1] 柯剑娟[1] 丁煌[1] 胡成俊 冯晓波[1] 王焱林[1] 李梦云[1] Chen Chao;Ke Jianjuan;Ding Huang;Hu Chengjun;Feng Xiaobo;Wang Yanlin;Li Mengyun(Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China;Department of Anatomy, Wuhan University School of Basic Medical Sciences, Wuhan 430071, Chin)

机构地区：[1]武汉大学中南医院麻醉科,430071 [2]武汉大学基础医学院人体解剖学教研室,430071

出　　处：《中华麻醉学杂志》2018年第3期292-295,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81471858）

摘　　要：目的探讨糖尿病因素对腺苷后处理减轻大鼠心肌缺血再灌注损伤的影响。方法成年雄性SD大鼠，体重230-260 g，采用高脂饲料喂养＋腹腔注射l%链脲佐菌素-柠檬酸盐缓冲液35 mg/kg制备2型糖尿病模型。取糖尿病模型制备成功的大鼠18只，采用随机数字表法分为3组（n＝6）：假手术组（DS组）、缺血再灌注组（DI/R组）和腺苷后处理组（DAP组）；另取非糖尿病大鼠18只，采用随机数字表法分为3组（n＝6）：假手术组（NS组）、缺血再灌注组（NI/R组）和腺苷后处理组（NAP组）。采用结扎冠状动脉左前降支30 min，再灌注2 h的方法制备心肌缺血再灌注损伤模型。于再灌注2 h时，采集股静脉血样，采用ELISA法测定血浆cTnI和CK-MB的浓度；然后处死大鼠，取心脏测定心肌缺血体积和心肌梗死体积。结果非糖尿病大鼠和糖尿病大鼠心肌缺血再灌注后血浆CK-MB和cTnI的浓度升高，心肌梗死体积百分比升高。腺苷后处理可显著降低非糖尿病大鼠和糖尿病大鼠血浆CK-MB和cTnI的浓度以及心肌梗死体积百分比（P〈0.05）。与NAP组比较，DAP组血浆CK-MB和cTnI的浓度升高，心肌梗死体积百分比升高（P〈0.05）。结论糖尿病因素可减弱腺苷后处理对大鼠的心肌保护作用。Objective To investigate the effect of diabetes mellitus （DM） on adenosine postconditioning-induced reduction of myocardial ischemia-reperfusion （I/R） injury in rats.Methods Adult male Sprague-Dawley rats, weighing 230-260 g, were used in the study.Type 2 DM was induced by high-fat diet and intraperitoneal l% streptozocin 35 mg/kg and confirmed by fasting blood glucose concentration〉16.7 mmol/L 72 h later.Eighteen rats with type 2 DM were divided into 3 groups （n=6 each） using a random number table： sham operation group （DS group）, I/R group （DI/R group） and adenosine postconditioning group （DAP group）. Eighteen healthy nondiabetic rats were selected and randomly divided into 3 groups （n=6 each）： sham operation group （NS group）, I/R group （NI/R group） and adenosine postconditioning group （NAP group）. Myocardial I/R was induced by 30 min occlusion of the left anterior descending branch of coronary artery followed by 2 h of reperfusion.Venous blood samples were collected from the femoral vein at 2 h of reperfusion for measurement of plasma cardiac troponin I （cTnI） and creatine kinase-MB （CK-MB） concentrations （by enzyme-linked immunosorbent assay）. The rats were then sacrificed immediately after blood sampling for determination of the myocardial ischemic area and infarct size.Results The plasma cTnI and CK-MB concentrations were significantly increased, and the percentage of myocardial infarct size was increased after myocardial I/R in nondiabetic and diabetic rats.Adenosine postconditioning significantly decreased plasma cTnI and CK-MB concentrations and percentage of myocardial infarct size in nondiabetic and diabetic rats （P〈0.05）. Compared with group NAP, the plasma concentrations of cTnI and CK-MB were significantly increased, and the percentage of myocardial infarct size was increased in group DAP （P〈0.05）.Conclusion DM can weaken cardioprotection induced by adenosine postconditioning in rats.

关 键 词：糖尿病 腺苷 缺血后处理 心肌再灌注损伤 

分 类 号：R614[医药卫生—麻醉学]