右美托咪定对术前睡眠剥夺大鼠术后痛的影响  

Effect of dexmedetomidine on postoperative pain in rats with preoperative sleep deprivation

作　　者：王宏伟[1] 艾艳秋[1] 李丽伟[1] 李治松[1] 张卫[1] Wang Hongwei;Ai Yanqiu;Li Liwei;Li Zhisong;Zhang Wei(Department of Anesthesiology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Chin)

机构地区：[1]郑州大学第一附属医院麻醉科,450052

出　　处：《中华麻醉学杂志》2018年第3期343-346,共4页Chinese Journal of Anesthesiology

摘　　要：目的评价右美托咪定对术前睡眠剥夺大鼠术后痛的影响。方法健康成年雄性SD大鼠50只，12-14周龄，体重200-300 g，采用随机数字表法将其分为5组（n＝10）：对照组（C组）、睡眠剥夺组（SD组）、切口痛组（IP组）、睡眠剥夺＋切口痛组（SD＋IP组）和睡眠剥夺＋切口痛＋右美托咪定组（SD＋IP＋DEX组）。采用小平台水环境法建立大鼠睡眠剥夺模型，于第1天睡眠剥夺完成后制备切口痛模型。SD＋IP＋DEX组切口痛造模前腹腔注射右美托咪定50 μg/kg，连续3 d，其余组腹腔注射等容量生理盐水。于术前或睡眠剥夺前1 d、术后或睡眠剥夺后12、24和72 h时测定机械痛阈和热痛阈；行为学测试完毕后，采集血样和脊髓背角组织，采用ELISA法测定血清皮质酮浓度，采用高效液相色谱法测定脊髓背角5-羟色胺（5-HT）含量。结果与C组比较，其余4组机械痛阈和热痛阈降低，血清皮质酮浓度及脊髓背角5-HT含量升高（P〈0.05）；与IP组比较，SD＋IP组机械痛阈和热痛阈降低，血清皮质酮及脊髓背角5-HT含量升高，SD＋IP＋DEX组机械痛阈和热痛阈升高（P〈0.05），血清皮质酮及脊髓背角5-HT含量差异无统计学意义（P〉0.05）；与SD＋IP组比较，SD＋IP＋DEX组机械痛阈和热痛阈升高，血清皮质酮及脊髓背角5-HT含量降低（P〈0.05）。结论右美托咪定可减轻术前睡眠剥夺大鼠术后痛，机制可能与抑制应激反应和脊髓背角5-HT水平有关。Objective To evaluate the effect of dexmedetomidine on postoperative pain in rats with preoperative sleep deprivation.Methods Fifty healthy adult male Sprague-Dawley rats, aged 12-14 weeks, weighing 200-300 g, were divided into 5 groups （n=10 each） using a random number table： control group （group C）, sleep deprivation group （group SD）, incisional pain group （group IP）, sleep deprivation plus incisional pain group （group SD＋ IP） and sleep deprivation plus incisional pain plus dexmedetomidine group （group SD＋ IP＋ DEX）. Sleep deprivation was induced by the flower pot technique, and then the incisional pain model was carried out on first day after completion of sleep deprivation.Dexmedetomidine 50 μg/kg was intraperitoneally injected for 3 consecutive days before establishing the model of incisional pain in group SD＋ IP＋ DEX, and the equal volume of normal saline was given in the other groups.The mechanical paw withdrawal threshold （MWT） and thermal paw withdrawal threshold （TWT） were measured before operation or at 1 day before sleep deprivation and after operation or at 12, 24 and 72 h after sleep deprivation.Blood samples were collected and spinal cord tissues were removed after the end of behavior test for determination of serum corticosterone concentrations （by enzyme-linked immunosorbent assay and content of 5-hydroxytryptamine （5-HT） in spinal dorsal horns （by high-performance liquid chromatography）.Results Compared with group C, the MWT and TWT were significantly decreased, and the serum corticosterone concentrations and content of 5-HT in spinal dorsal horns were increased in the other 4 groups （P〈0.05）. Compared with group IP, the MWT and TWT were significantly decreased, and the serum corticosterone concentrations and content of 5-HT in spinal dorsal horns were increased in group SD＋ IP, and the MWT and TWT were significantly increased （P〈0.05）, and no significant change was found in the serum corticosterone concentrations or content of

关 键 词：右美托咪啶 睡眠剥夺 疼痛 手术后 

分 类 号：R614[医药卫生—麻醉学]