室旁核小胶质细胞在慢性内脏痛大鼠抑郁易感性中的作用  

Role of microglia in paraventricular nucleus in susceptibility to depression in rats with chronic visceral pain

作　　者：陈祖萍[1] 孙杰[2] 高操[1] 沈江[1] 陈自洋 Chen Zuping;Sun Jie;Gao Cao;Shen Jiang;Chen Ziyang(Department of Anesthesiology, First People′s Hosptial of Changzhou, Changzhou 213003, China;Department of Anesthesiology, Jiangsu Province Hospital, Nanjing 210009, China)

机构地区：[1]常州市第一人民医院麻醉科,213003 [2]江苏省人民医院麻醉科,南京市210009

出　　处：《中华麻醉学杂志》2018年第3期347-350,共4页Chinese Journal of Anesthesiology

摘　　要：目的评价室旁核小胶质细胞在慢性内脏痛大鼠抑郁易感性中的作用。方法清洁级健康雄性SD大鼠96只，8日龄，采用随机数字表法分为6组（n＝16）：假手术组（S组）、慢性内脏痛组（CHVP组）、假手术＋结直肠扩张组（S＋C组）、慢性内脏痛＋结直肠扩张组（CHVP＋C组）、慢性内脏痛＋PBS＋结直肠扩张组（CHVP＋P＋C组）、慢性内脏痛＋米诺环素＋结直肠扩张组（CHVP＋M＋C组）。S组大鼠不进行结直肠扩张；CHVP组大鼠于出生后第8、10和12天，通过每天给予2次结直肠扩张制备大鼠慢性内脏痛模型；出生后第8周，CHVP＋P＋C组通过立体定位法室旁核注射PBS 0.5 μl，CHVP＋M＋C组注射2%米诺环素0.5 μl。2 h后每组随机取8只大鼠测定内脏痛痛阈。S＋C组、CHVP＋C组、CHVP＋P＋C和CHVP＋M＋C组结直肠扩张2次，随后行旷场实验和糖水偏好实验，处死大鼠取室旁核，免疫荧光法检测小胶质细胞活化情况。结果与S组和S＋C组相比，CHVP组、CHVP＋C组、CHVP＋P＋C组和CHVP＋M＋C组痛阈降低（P〈0.05）；与CHVP＋P＋C组相比，CHVP＋M＋C组痛阈升高（P〈0.05）；与S组、CHVP组和S＋C组相比，CHVP＋C组、CHVP＋P＋C组和CHVP＋M＋C组总运动距离、直立次数和糖水消耗率减少，室旁核小胶质细胞活化率升高（P〈0.05）；与CHVP＋P＋C组相比，CHVP＋M＋C组总运动距离、直立次数和糖水消耗率增加，室旁核小胶质细胞活化率降低（P〈0.05）。 结论室旁核小胶质细胞参与了慢性内脏痛大鼠抑郁易感性的调节。Objective To evaluate the role of microglia in paraventricular nucleus （PVN） in susceptibility to depression in rats with chronic visceral pain.Methods Ninety-six pathogen-free healthy male Sprague-Dawley rats, aged 8 days, were divided into 6 groups （n=16 each） using a random number table： sham operation group （S group）, chronic visceral pain group （CHVP group）, sham operation plus colorectal distension group （S＋ C group）, chronic visceral pain plus colorectal distension group （CHVP＋ C group）, chronic visceral pain plus phosphate buffer solution plus colorectal distension group （CHVP＋ P＋ C group） and chronic visceral pain plus minocycline plus colorectal distension group （CHVP＋ M＋ C group）. Colorectal distension was not performed in S group.In CHVP group, chronic visceral pain was induced by performing colorectal distension twice daily on postnatal days 8, 10, and 12.Phosphate buffer solution 0.5 μl was injected into PVN by stereotaxic method at 8th week after birth in CHVP＋ P＋ C group, and 2% minocycline 0.5 μl was injected into PVN at 8th week after birth in CHVP＋ M＋ C group.Eight rats in each group were selected 2 h later for measurement of visceral pain threshold.In S＋ C, CHVP＋ C, CHVP＋ P＋ C and CHVP＋ M＋ C groups, colorectal distension was performed for 2 times, open field test and sucrose preference test were then performed, the rats were sacrificed and PVN was removed for determination of microglial activation by immunofluorescence.Results The pain threshold was significantly decreased in CHVP, CHVP＋ C, CHVP＋ P＋ C and CHVP＋ M＋ C groups as compared with S and S＋ C groups （P〈0.05）. The pain threshold was significantly increased in CHVP＋ M＋ C group when compared with CHVP＋ P＋ C group （P〈0.05）. Compared with S, CHVP and S＋ C groups, the total locomotor distance, the number of rearing and sucrose consumption were significantly reduced, and the proportion of activated microglia in PVN was increased in CHVP＋ C,

关 键 词：下丘脑室旁核 小神经胶质细胞 内脏 疼痛 抑郁 疾病易感性 

分 类 号：R614[医药卫生—麻醉学]