带鱼酶解蛋白亚铁螯合物对泥鳅免疫特性的影响  

Effect of ferrous chelate Trichiurus lepturus protein on the immune characteristics of Misgurnus anguillicaudatus

作　　者：林慧敏 袁宁 李颖杰 邓尚贵 张宾 霍健聪 LIN Huimin;YUAN Ning;LI Yingjie;DENG Shanggui;ZHANG Bin;HUO Jiancong(College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, China)

机构地区：[1]浙江海洋大学食品与医药学院,浙江舟山316000

出　　处：《水产学报》2018年第6期968-974,共7页Journal of Fisheries of China

基　　金：国家自然科学基金(31471609);浙江省自然科学基金(LY18C200007;LY18C200008;LY17C200011)~~

摘　　要：为探究带鱼酶解蛋白亚铁螯合肽对泥鳅免疫特性的影响及其在泥鳅体内的代谢分布情况,通过在泥鳅饲料中添加不同比例带鱼酶解蛋白亚铁螯合肽,喂养40 d,检测泥鳅消化酶活性、血清和肝脏生理生化指标,运用活体成像技术检测FITC标记的带鱼酶解亚铁螯合肽在泥鳅体内的分布。结果显示,亚铁螯合肽添加量为2 g/kg时,脂肪酶活性极显著高于对照组;添加量为1 g/kg时,淀粉酶和胃蛋白酶活性显著高于正常对照组;各实验组胰蛋白酶活性相比于对照组无显著性差异。血清指标中超氧化物歧化酶和溶菌酶在添加量为2 g/kg时,活性显著高于正常对照组;相对与正常对照组,过氧化氢酶在添加量为1 g/kg表现为显著性差异,2 g/kg的添加量表现为极显著性差异;丙二醛在添加量为2 g/kg显著低于正常对照组。活体成像技术发现FITC标记的带鱼酶解蛋白亚铁螯合肽会较长时间滞留在泥鳅体内,荧光强度主要集中在泥鳅身体上部,推测带鱼酶解蛋白亚铁螯合肽被泥鳅吸收后主要分布在肝脏、胆囊等器官内。研究表明,饲料中添加1~2g/kg的带鱼酶解蛋白亚铁螯合肽能提高养殖泥鳅的消化道酶活性,增强泥鳅的非特异性免疫。The test of the digestive enzyme activity, observation of intestinal tissue and the living imaging technology were applied to investigate the effect of the ferrous chelating peptide on the digestive tract. The test of indexes of biochemical indexes of serum and liver, and observation of the liver were used to investigate the effect of the ferrous chelating peptide on immunological characteristics of Misgurnus anguillicaudatus. The result showed lipase activity was significantly higher than that of the control group when the ferrous chelating peptide was 2 g/kg; amylase activity of pepsin was significantly higher than the normal control group when the ferrous chelating peptide was 1 g/kg; trypsin activity in each experimental group compared with the control group had no significant difference. SOD and lysozyme in serum were significantly higher than those of normal control group when the ferrous chelating peptide was 2 g/kg; compared with the normal control group, CAT at ferrous chelating peptide concentration of 1 g/kg showed significant difference. Compared with control group, the morphological structure of each experimental group had no change. With the application of living imaging technology, complexes of ferrous chelating peptide with FITC marked stayed in M. anguillicaudatus for a long time and fluorescence intensity was mainly enriched in the upper body of M. anguillicaudatus, hence, after absorption, the ferrous chelating peptide was speculated to be distributed in the liver, gallbladder and other organs. The results showed that the hydrolysis of ferrous chelate peptide could improve the digestive enzyme activities of loach and enhance non-specific immunity of loach.

关 键 词：带鱼 泥鳅 亚铁螯合肽 消化道酶 免疫 

分 类 号：S963.7[农业科学—水产养殖]