巨噬细胞和肺微血管内皮细胞对烟曲霉作用的研究  

Effect of macrophages and pulmonary microvascular endothelial cells on Aspergillus fumigatus

作　　者：宋珺[1] 韩菁[1] 孙越[1] 施伟民[1] SONG Jun;HAN Jing;SUN Yue;SHI Weimin(Department of Dermatology, Shanghai First People＇s Hospital, Shanghai Jiaotong University, Shanghai 200080, Chin)

机构地区：[1]上海交通大学附属第一人民医院皮肤科,上海200080

出　　处：《中国医药导报》2018年第13期18-20,F0003,F0004,共5页China Medical Herald

基　　金：国家重点基础研究发展计划(973计划)(2013CB531604)

摘　　要：目的探讨巨噬细胞和肺微血管内皮细胞对烟曲霉作用。方法构建小鼠巨噬细胞株J774细胞烟曲霉感染的体外模型和人肺微血管内皮细胞(HPMVECs)烟曲霉感染的体外模型,利用激光共聚焦扫描显微镜观察共培养体系。结果 J774细胞对烟曲霉具吞噬作用,但在共培养24 h的动态观察中,并未观察到HPMVECs内化烟曲霉,仅观察到烟曲霉的感染导致HPMVECs形态的变化和细胞完整性的破坏。结论 J774细胞对烟曲霉具吞噬作用,HPMVECs无内化烟曲霉的作用,烟曲霉感染可导致HPMVECs形态的变化和细胞完整性的破坏。Objective To investigate effect of macrophages and pulmonary microvascular endothelial cells（HPMVECs）on Aspergillus fumigatus. Methods In vitro models of Aspergillus fumigatus-treated HPMVECs and Aspergillus fumigatus-treated J774 cells were established, confocal laser scanning microscope was used for detection of co-cultures. Results Aspergillus fumigatus were phagocyted by J774 cells. However, Aspergillus fumigatus were not internalized by HPMVECs, onlu morphology change and integrity compromise were observed on Aspergillus fumigatus-treated HPMVECs. Conclusion J774 cells phagocytes Aspergillus fumigatus. HPMVECs can＇t internalize Aspergillus fumigatus.Aspergillus fumigatus can cause morphology change and integrity compromise in HPMVECs.

关 键 词：巨噬细胞 肺微血管内皮细胞 烟曲霉 

分 类 号：R563[医药卫生—呼吸系统]