含白芷方剂组方规律及核心药对“白芷-川芎”分子机制的研究  

Compatibility law of Baizhi formulae and molecular mechanism of core herbal pair "Baizhi-Chuanxiong"

作　　者：苏瑾 唐仕欢[1] 郭非非 李德凤[1] 张毅[1] 许海玉[1] 杨洪军[1] SU Jin;TANG Shi-huan;GUO Fei-fei;LI De-feng;ZHANG Yi;XU Hai-yu;YANG Hong-jun(Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China)

机构地区：[1]中国中医科学院中药研究所

出　　处：《中国中药杂志》2018年第7期1331-1337,共7页China Journal of Chinese Materia Medica

基　　金：国家科技支撑计划项目(2011BAI07B08);国家自然科学基金面上项目(81473414)

摘　　要：该研究先借助中医传承辅助平台,对含白芷方剂的组方规律进行分析,获得核心组合药对＂白芷-川芎＂,并对＂白芷-川芎＂的临床用药规律进行系统分析,然后运用整合药理学思维对核心组合药对＂白芷-川芎＂治疗头痛的分子机制进行探讨,旨在发现白芷的配伍规律及其干预头痛的分子机制,为白芷及其组方的后续研究提供理论依据及参考。《中医方剂大词典》共收录含白芷方剂3 887种,涉及中药2 534味,将支持度设置为20%进行分析,共得到常用配伍组合16个,主治疾病15种,白芷常应用于治疗头痛,核心组合＂白芷-川芎＂在头痛的治疗中亦较为常用,与古籍记载相吻合。在整合药理学平台中建立＂白芷-川芎＂化学数据库,检索并添加头痛、偏头痛相关疾病靶标,构建＂成分-靶标-通路＂核心网络图,对＂白芷-川芎＂治疗头痛的分子机制进行研究。结果发现,＂白芷-川芎＂核心组合有37个化学成分与头痛治疗密切相关,这些成分可通过调节5-羟色胺,或作用于炎症相关靶标及嘌呤代谢、丙酮酸代谢、脂肪酸降解、碳代谢、糖异生等多条能量代谢有关通路来达到治疗头痛的目的。By using the traditional Chinese medicine inheritance support system（ TCMISS） in this study,the prescription rules of Baizhi formulae were analyzed and the core herbal pair ＂ Baizhi-Chuanxiong＂ was obtained. Through the systemic analysis of prescription rules of ＂ Baizhi-Chuanxiong＂ and combined with the pharmacology thinking of ＂ Baizhi-Chuanxiong＂ in treating headache,the paper was aimed to find out the combination rules containing Baizhi andits molecular mechanisms for treating headaches,and provide the theory basis for further research and reference of Baizhi and its formula. Totally 3 887 prescriptions were included in this study,involving 2 534 Chinese herbs. With a support degree of 20% in analysis,16 most commonly used drug combinations were screened,which were mainly used to treat 15 types of diseases. Baizhi was often used to treat headache,and the core combination ＂ Baizhi-Chuanxiong＂ was also often used to treat,consistent with ancient record. A chemical database was established; then the headache and migraine disease targets were retrieved and added in the database to build up the ＂ compounds-targets-pathways ＂ core network of ＂ Baizhi-Chuanxiong＂ by the internetbased computation platform for IP of TCM（ TCM-IP）. TCM-IP was then applied to study the molecular mechanism of ＂ Baizhi-Chuanxiong＂ treatment of headache. The results suggested that37 chemical compounds in the core combination ＂ Baizhi-Chuanxiong＂ were closely related with headache treatment by adjusting serotonin levels or applying to inflammation-related targets and energy metabolism pathways such as purine metabolism,pyruvate metabolism,fatty acid degradation,carbon metabolism and gluconeogenesis.

关 键 词：白芷 配伍规律 整合药理学 分子机制 头痛 

分 类 号：R289[医药卫生—方剂学]