不动杆菌3-59中群体感应信号分子淬灭酶基因克隆及其生物信息学分析  

Identification and bioinformatics analysis of quorum-quenching genes fromAcinetobacter sp.3-59

作　　者：张清霞[1] 张迎 何玲玲[1] 蒋天萍 童蕴慧[1] ZHANG Qingxia;ZHANG Ying;HE Lingling;JIANG Tianping;TONG Yunhui(College of Horticultural and Plant Protection, Yangzhou University, Yangzhou 225009, Chin)

机构地区：[1]扬州大学园艺与植物保护学院,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2018年第1期105-110,共6页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(31772210);江苏省重点研发计划(现代农业)项目(BE2017344);扬州大学科技创新培育基金项目(2016CXJ066)

摘　　要：N-酰基高丝氨酸内酯(AHL)在许多植物病原细菌中作为群体感应信号分子调控致病因子的表达。在淬灭群体感应系统中,AHL可控制依赖于群体感应的植物细菌病害。以实验室保存的4个群体淬灭酶产生菌为研究对象,对β-半乳糖苷酶活性进行测定,结果表明菌株3-59对信号分子降解能力最强。根据16SrDNA相似性,将该菌株鉴定为不动杆菌(Acinetobactersp.)3-59。采用PCR法从菌株3-59中克隆到AHL淬灭酶基因aciJ,该基因全长1 491bp,编码496个氨基酸,预测分子量约为55.69ku,估测等电点为9.52。氨基酸序列比对结果显示,其与不动杆菌的单加氧酶相似性在90%以上。AciJ蛋白有跨膜结构域,N端无信号肽,有1个糖基化位点位于285位,有43个磷酸化位点均匀分布于整个多肽链中。N-acylhomoserine lactones（AHLs）used as quorum-sensing signaling molecules regulated expressions of viurlences in many phytopathogenic bacteria.Therefore,quorum quenching（QQ）will treat infections caused by quorum sensing-dependent pathogenic bacteria.This study assayedβ-galactosidase activities of four QQ bacteria stored in our laboratory.The results showed that strain 3-59 had the strongest AHL-degrading ability and designated Acinetobacter sp.based on 16 SrDNA gene sequence identity.The gene,named aciJ,responsible for AHL-degrading was cloned by PCR amplification.Sequence analysis revealed that the ORF was 1 491 bp,coding for 496 amino acids with a predicted molecular mass of 55.69 ku and pI value of 9.52.The predicted amino acid sequence of AciJ shares over 90%identity to monooxygenases fromAcinetobacter spp..Bioinformatics analysis showed that there was a possible transmembrane domain but no possible signal peptide in the N terminal of the peptide.There was a glycosylation site which located 285 and 43 phosphorylation sites which uniformly distributed throughout the polypeptide chain.

关 键 词：不动杆菌 群体感应淬灭酶 酰基高丝氨酸内酯 单加氧酶 

分 类 号：Q939.1[生物学—微生物学]