分子修饰狂犬病ERA疫苗株剂量依赖反应及对强毒的攻毒保护研究  

Investigation of dose-dependent immune response and protection of the modified ERA strain rabies vaccine against the challenge of rabies virus strains

作　　者：王雪莲[1] 李翠霞 帅磊[1] 王喜军[1] 葛金英[1] 步志高[1] WANG Xue-lian;LI Cui-xia;SHUAI Lei;WANG Xi-jun;GE Jin-ying;BU Zhi-gao(State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China)

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150069

出　　处：《中国预防兽医学报》2018年第5期421-425,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：狂犬病犬瘟热二联活载体疫苗项目(0302012004)

摘　　要：为评价分子修饰狂犬病ERA疫苗株(简称rERAG_(333E))的免疫效果,本实验将其分别以10~3FFU/mL、10~4FFU/m L、10~5FFU/mL、10~6FFU/mL的剂量口服免疫小鼠,监测小鼠血清中和抗体持续期;同时在小鼠免疫4周后采用不同现地流行株进行攻毒保护实验。结果表明,rERAG_(333E)以10~4FFU/mL以上剂量免疫小鼠,免疫后各实验组小鼠均能够产生较高水平的抗狂犬病病毒(RABV)中和抗体,且能够抵抗经典效检强毒株CVS-24和现地流行强毒株GX/09和MRV的致死性攻击,保护率达到100%;免疫有效持续期能够达到24周以上。以上结果表明rERAG_(333E)是一种安全有效,具有推广使用潜力的狂犬病口服疫苗候选株。To evaluate the immune efficiency of the modified ERA strain rabies vaccine （rERAG333E）, groups of mice were oral immunized with rERAG333E from the doses of 10^3 FFU/mL to 10^6 FFU/mL, and the duration of virus neutralizing antibody （VNA） and protection in mice were detected against the challenging with different genotype rabies virus （RABV） strains at 4 weeks post immunization. The results showed when mice were immunized with more than l04 FFU/mL doses of the rERAG333E, the mice produced high levels of VNA against RABV which persisted more than 24 weeks, and the mice were completely protected from the challenge of classical virulent virus CVS-24 and epidemic virulent virus GX/09 and MRV. These results indicated that the rERAG333E had the potential to serve as a safe and effective oral RABV vaccine strain.

关 键 词：剂量依赖反应 口服疫苗 狂犬病 ERA 

分 类 号：S852.65[农业科学—基础兽医学]