鱼藤酮乳油中鱼藤酮含量的反相HPLC-DAD法测定  

Determination of Rotenone in Rotenone Emulsifiable Concentrate by RP-HPLC-DAD

作　　者：王金林[1] 翁盼伟 巫双丽 周靖[1,2] 崔淑芬 WANG Jinlin;WENG Panwei;WU Shuangli;ZHOU Jing;CUI Shufen(School of Applied Chemistry and Biotechnology, Shenzhen Polytechnic, Shenzhen, Guangdong 518055, China, 2. Guangdong Litchi Longan Pest Control Science and Technology Innovation Center, Shenzhen, Guangdong 518055, China)

机构地区：[1]深圳职业技术学院应用化学与生物技术学院,广东深圳518055 [2]广东省荔枝龙眼病虫防控科技创新中心,广东深圳518055

出　　处：《深圳职业技术学院学报》2018年第3期33-36,共4页Journal of Shenzhen Polytechnic

基　　金：深圳职业技术学院科技计划重点资助项目(601722K27013)

摘　　要：建立反相HPLC-二极管阵列检测器(diode array detector,DAD)法测定了鱼藤酮乳油中鱼藤酮的含量.色谱柱为Agilent Zorbax Eclipse XDB C18(250 mm×4.6 mm,5μm);流动相为甲醇-超纯水(70:30);采用等浓度洗脱,流速为1.0 m L/min;柱温35℃;检测波长297 nm;进样体积10μL.该方法的线性范围是1~200μg/m L(R﹥0.999),最低检出浓度(LOD,S/N=3)为0.1μg/m L,定量限(LOQ,S/N=10)为0.25μg/m L,重复性实验中鱼藤酮峰面积的相对标准偏差(RSD,n=5)为2.51%,加标回收率为97.14%~98.11%.A reversed phase HPLC- diode array detector （DAD） method was developed for the determination of rotenone in rotenone emulsifiable concentrates. The RP-HPLC-DAD was performed on a Agilent Zorbax Eclipse XDB C 18 column （250 mm×4.6 mm, 5 μm） , and the isocratic gradient elution mobile phases were methanol and water （70：30）. The flow rate was 1 mL/min and the column temperature was 35℃. The detection wave length was 297 mm and the injection volume was 10 μL. A good linearity was obtained over the concentration range of 1-200 μg/mL （R 〉 0.999）. The limit of detection （LOD, S/N = 3） and the limit of quantitation （LOQ, S/N = 10） are 0.1 μg/mL and 0.25 μg/mL, respectively. The relative standard deviation of peak areas ofrotenone （RSD, n=5） is 2.51% and the recovery rate are 97：14 % - 98.11%.

关 键 词：鱼藤酮乳油 鱼藤酮 HPLC-DAD 峰纯度 方法验证 

分 类 号：TQ453.299[化学工程—农药化工]