急性喉炎患儿肺炎支原体和EB病毒感染与T淋巴细胞亚群的关系分析  

Prevalence of Mycoplasma pneumoniae and Epstein-Barr virus infections in children with acute laryngitis and its relationship with T lymphocyte subsets

作　　者：黄邀[1] 阮奕[2] 吴惠兰[1] 陆卫民[1] 周芳芳 HUANG Yao;RUAN Yi;WU Hui-lan;LU Wei-min;ZHOU Fang-fang(Taizhou Hospital of Zhejiang Province, Taizhou , Zhejiang 317000, China)

机构地区：[1]浙江省台州医院儿内科,浙江台州317000 [2]浙江省台州医院检验科,浙江台州317000

出　　处：《中华医院感染学杂志》2018年第10期1555-1558,共4页Chinese Journal of Nosocomiology

基　　金：浙江省医药卫生一般研究计划基金资助项目(2015KYB421)

摘　　要：目的了解儿童急性喉炎患者肺炎支原体和EB病毒感染情况,及其与T淋巴细胞亚群的关系。方法选择医院2011年1月-2017年4月406例急性喉炎住院患儿作为急性喉炎病例组,另选406名健康体检儿童作为对照组;儿童急性喉炎患者根据是否感染肺炎支原体分为肺炎支原体感染组和肺炎支原体未感染组,根据是否感染EB病毒分为EB病毒感染组和EB病毒未感染组,采用流式细胞仪测定外周血淋巴细胞CD_3^+、CD_4^+、CD_8^+百分比。结果 406例急性喉炎住院患儿中,发生肺炎支原体感染89例,占21.9%,发生EB病毒感染40例,占9.9%;急性喉炎病例组患儿CD_8^+百分比低于对照组(t=17.364,P<0.001),CD_4^+/CD_8^+比值高于对照组(t=4.893,P<0.05),两组CD_3^+、CD_4^+百分比比较差异无统计学意义;肺炎支原体感染组和肺炎支原体未感染组CD_3^+、CD_4^+、CD_8^+、CD_4^+/CD_8^+数值比较差异无统计学意义;EB病毒感染组和EB病毒未感染组CD_3^+、CD_4^+、CD_8^+、CD_4^+/CD_8^+数值比较差异无统计学意义。结论急性喉炎患儿常伴有肺炎支原体和(或)EB病毒感染,并导致CD_8^+百分比的降低,CD_4^+/CD_8^+比值的升高。OBJECTIVE To investigate the prevalence of Mycoplasma pneumoniae and Epstein-Barr（EB）virus infections in children with acute laryngitis and analyze its relationship with T lymphocyte subsets.METHODS Totally406children with acute laryngitis who were hospitalized from Jan 2011to Apr 2017were chosen as the acute laryngitis group,and 406healthy children who received physical examination were set as the control group.The children with acute laryngitis were divided into the M.pneumoniae infection group and the non-M.pneumoniae infection group according to the status of M.pneumoniae infections and were divided into the EB virus infection group and the non-EB virus infection group according to the status of EB virus infections.The percentages of lymphocytes CD3＋,CD4＋and CD8＋in peripheral blood were determined by using flow cytometry.RESULTS Of the 406hospitalized children,89（21.9%）had M.pneumoniae infections,and 40（9.9%）had EB virus infections.The percentage of CD8＋of the acute laryngitis group was significantly lower than that of the control group（t=17.364,P0.001）,the ratio of CD4＋to CD8＋of the acute laryngitis group was significantly higher than that of the control group（t=4.893,P〈0.05）,and there were no significant differences in the percentages of CD3＋and CD4＋between the two groups;there were no significant differences in the percentages of CD3＋,CD4＋and CD8＋as well as the ratio of CD4＋to CD8＋between the M.pneumoniae infection group and the non-M.pneumoniae infection group;there were no significant differences in the percentages of CD3＋,CD4＋and CD8＋as well as the ratio of CD4＋to CD8＋between the EB virus infection group and the non-EB virus infection group.CONCLUSIONThe children with acute laryngitis are usually complicated with M.pneumoniae and EB virus infections,which may lead to the reduction of percentage of CD8＋and the increase of the ratio of CD4＋to CD8＋.

关 键 词：急性喉炎 儿童 肺炎支原体 EB病毒 T淋巴细胞 

分 类 号：R767.11[医药卫生—耳鼻咽喉科]