基于层次分析和正交设计法优选白龙清热胶囊的提取工艺  

Optimization of extraction process of Bailong Qingre capsules based on orthogonal design and analytic hierarchy process

作　　者：陈旭 陈丹[2] 宋洪涛[1] CHEN Xu;CHEN Dan;SONG Hong-tao(Department of Pharmacy, Fuzhou General Hospital, Fujian Fuzhou 350025, China;Fujian University of Traditional Chinese Medicine, Fujian Fuzhou 350122, China)

机构地区：[1]福州总医院药学科,福建福州350025 [2]福建中医药大学药学院,福建福州350122

出　　处：《中国医院药学杂志》2018年第10期1059-1064,1069,共7页Chinese Journal of Hospital Pharmacy

基　　金：军队医疗机构制剂标准提高科研专项课题(编号:14ZJZ17)

摘　　要：目的:优选白龙清热胶囊处方药材的最佳提取工艺。方法:建立高效液相色谱(HPLC)法同时测定处方药味中龙胆苦苷、桑皮苷A、栀子苷、秦皮乙素、蒙花苷、甘草苷等指标性成分的含量。采用UV法测定总黄酮含量。以各指标性成分的提取率、总黄酮含量和浸膏得率作为评价指标。采用正交试验设计法,考察乙醇浓度、乙醇用量、提取时间、浸泡时间对处方原料药材提取效果的影响;采用层次分析法确定权重系数,综合评价实验结果。结果:建立了以龙胆苦苷、桑皮苷A、栀子苷、秦皮乙素、蒙花苷、甘草苷为指标性成分的HPLC同步测定方法;6种成分分别在7.36~73.60、0.96~9.60、9.28~92.80、4.64~46.40、2.24~22.40、0.8~8.0μg·mL^(-1)浓度范围内呈良好的线性关系(r为0.999 7~0.999 9),加样回收率为99.31%~102.1%,RSD均小于3.0%;龙胆苦苷、桑皮苷A、总黄酮、栀子苷、秦皮乙素、蒙花苷、甘草苷、干膏率的权重系数分别为0.250 1,0.250 1,0.160 1,0.098 3,0.098 3,0.098 3,0.027 9,0.016 9;综合评价后确定的白龙清热胶囊最佳提取工艺条件为用70%乙醇为提取溶剂,药液比为1∶10,浸泡时间2 h,提取2次,每次2 h。结论:优化的提取工艺方法可行、工艺稳定、重复性好,保证了白龙清热胶囊处方中各味药材中有效成分的提取,可适用于工业生产。OBJECTIVE To optimize the best extraction process of prescription herbs of Bailongqingre capsules.METHODS Gentiopicroside,mulberroside A,geniposide,aesculetin,linarin and liquiritin in the prescription herbs were simultaneously determined by HPLC.The content of total flavonoids was determined by UV method.The extraction rate of each index component,total flavonoid content and extract yield were taken as the evaluation indexes.Orthogonal test was used to optimize the ethanol extraction technology by taking ethanol concentration,the amount of ethanol,extraction time and soaking time.The weight coefficient was determined by AHP,and the experimental results were evaluated synergistically.RESULTS The HPLC method was established for the determination of gentiopicroside,mulberroside A,geniposide,aesculetin,linarin and liquiritin.The excellent linearity was obtained with correlation coefficients of 7.36-73.60,0.96-9.60,9.28-92.80,4.64-46.40,2.24-22.40 and 0.8-8.0μg·mL-1,r of 0.999 7-0.999 9,and the average recoveries of the six compounds were 99.31%-102.08%with all RSD values less than 3.0%.The weight coefficient of gentiopicroside was 0.250 1,mulberroside A was 0.250 1,total flavonoids was 0.1601,geniposide was 0.098 3,aesculetin was 0.098 3,linarin was 0.098 3,liquiritin was 0.027 9,and dry extract was 0.016 9.According to the comprehensive evaluation,the optimum extraction conditions were as follows：soaking time2 hours,decocting twice for 2 hours each by ten times of 70% ethanol.CONCLUSION The optimized extraction technique is feasible,stable and repeatable,and facilitates to extract the effective components of Bailong Qingre capsules and suits for industrial production.

关 键 词：白龙清热胶囊 提取工艺 正交试验设计 层次分析法 

分 类 号：R927.2[医药卫生—药学]