蛭龙活血通瘀胶囊对心肌梗死模型大鼠外周血CD34^+、CD133^+的影响  

Effect of Zhilong Huoxue Tongyu Capsule on Peripheral Blood CD_(34)^+ and CD_(133)^+ for Myocardial Infarction Model Rats

作　　者：李长江 徐奎 李小林 杨思进 LI Changfiang;XU Ku;LI Xiaolin;et al.(Southwest Medical University （ Luzhou Sichuan 646000, China;Department of Cardioencephalopathy, Traditional Chinese Medicine Hospital Attached to Southwest Medical University （ Luzhou Sichuan 646000, China)

机构地区：[1]西南医科大学,四川泸州646000 [2]西南医科大学附属中医医院心脑病科,四川泸州646000

出　　处：《四川中医》2018年第5期45-49,共5页Journal of Sichuan of Traditional Chinese Medicine

基　　金：泸州医学院青年基金项目(编号:2011~309)

摘　　要：目的:通过检测心肌梗死模型大鼠血清中CD34^+、CD133^+的含量,探索蛭龙活血通瘀胶囊对心肌梗死大鼠骨髓的内皮祖细胞(endothelial progenitor cells EPCs)动员的影响以及可能的机制。方法:健康SD大鼠48只,随机分为假手术组、卡托普利组、模型组、蛭龙活血通瘀胶囊高剂量组(简称蛭龙高剂量组)、蛭龙活血通瘀胶囊中剂量组(简称蛭龙中剂量组)、蛭龙活血通瘀胶囊低剂量组(简称蛭龙低剂量组),每组8只。结扎冠脉前降支复制急性心肌梗死大鼠模型。应用蛭龙活血通瘀胶囊高中低剂量、卡托普利灌胃3周。各组大鼠于术后第0天、第1天、第3天、第5天、第7天、第20天尾静脉采血法取静脉血0.5~1ml,流式细胞仪分别检测血清中CD34^+、CD133^+的含量。处死大鼠,心脏直接采血3~5ml,分离出单核细胞(monocyte MNCs)层,培养4天,观察内皮祖细胞的形态。结果:血清中CD34^+、CD133^+的含量:与假手术组比较,各观察点模型组、卡托普利组、蛭龙高剂量组、蛭龙中剂量组、蛭龙低剂量组血清中CD34^+、CD133^+的含量均升高,差异具有统计学意义(P<0.001);与模型组比较,各观察点卡托普利组、蛭龙高剂量组、蛭龙中剂量组、蛭龙低剂量组血清中CD34^+、CD133^+含量均升高,差异具有统计学意义(P<0.001);与卡托普利组比较,各观察点蛭龙高剂量组血清中CD34^+、CD133^+含量无明显变化,差异无统计学意义(P>0.05);与蛭龙中剂量组比较,各观察点卡托普利组、蛭龙高剂量组血清中CD34^+、CD133^+含量均升高,差异具有统计学意义(P<0.001);与蛭龙低剂量组比较,各观察点卡托普利组、蛭龙高剂量组、蛭龙中剂量组血清中CD34^+、CD133^+含量均升高,差异具有统计学意义(P<0.001)。培养四天后,EPCs细胞体积较前变大,部分呈椭圆形,数量变多,多数贴壁生长。结论:(1)蛭龙活血通瘀胶囊能够有效促进大模型大鼠心肌梗死后EPCs向外周血Objective： By detecting myocardial infarction rats serum CD34^＋,CD133^＋ content,exploring Zhilong Huoxue Capsule on myocardial infarction in rats bone marrow endothelial progenitor cell mobilization effects and possible mechanism. Methods： 48 Healthy SD rats were randomly divided into controlled group,captopril group,model group,Zhilong Huoxue Capsule high-dose group,Zhilong Huoxue Capsule middle-dose group,Zhilong Huoxue Capsule low-dose group,each group had eight rats. Coronary artery ligation of copying the rat model of acute myocardial infarction（ AMI）. Control line open thoracic surgery,broke the pericardium and exposed to the heart,not ligation of coronary artery; Application of high,medium and low dose of Zhilong Huoxue Tongyu Capsule,captopril gavage for 3 weeks. The controlled group,the model group were amount of saline lavage for three weeks. Groups of rats in postoperative 0 day,1 day,3 days,5 days,7 days,20 days caudal vein blood sampling method in venous blood 0. 5 - 1 ml,flow cytometry instrument in serum was detected in the content of CD34^＋,CD133^＋ ; Put to death in the rat,the heart blood directly for 3 - 5 ml,isolate mononuclear cells layer,cultivating four days,observing the endothelial progenitor cells. Results： In serum of CD34^＋、CD133^＋ content： compared with the controlled group,each observation point model group,captopril group,Zhilong high-dose group,Zhilong middle-dose group,Zhilong low-dose group serum content of CD34^＋,CD133^＋ were higher,the difference was statistically significant（ P〈 0. 001）; Compared with model group,the observation point the captopril group,Zhilong high-dose group,Zhilong middle-dose group,Zhilong low-dose group CD34^＋、CD133^＋ were higher,statistically significant difference（ P〈 0. 001）; In comparison with the captopril group there was no significant change in the CD34^＋,CD133^＋ content in the Zhilong high-dose group serum of the level,and the difference was not statistically significant（ P〉0. 05）. In compa

关 键 词：蛭龙活血通瘀胶囊 急性心肌梗死 内皮祖细胞 CD34抗原 CD133抗原 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]