栎属近缘种指纹图谱构建及遗传结构  

Construction of SSR fingerprint and research of genetic structure in relative Quercus species

作　　者：郭斌[1] Guo Bin(Shanxi Academy of Forest Sciences, Taiyuan 030012, Shanxi, China)

机构地区：[1]山西省林业科学研究院,山西太原030012

出　　处：《北京林业大学学报》2018年第5期10-18,共9页Journal of Beijing Forestry University

基　　金：山西常绿树种引种选育及快繁研究(20140312001);国家林木(含竹藤花卉)种质资源平台建设与运行服务(2005DKA21003)

摘　　要：【目的】对栎属近缘种质进行指纹图谱构建,并分析其遗传结构,为栎属近缘种鉴定及分类提供了重要工具。【方法】以23份栎属近缘种质为研究对象,利用遗传背景差异大的4份种质进行SSR引物筛选,选出9对扩增条带清晰、具有多态性且重复性好的引物对其进行扩增,利用毛细管电泳技术对PCR荧光产物进行检测,采用引物-分子量组合法构建23份种质的指纹图谱,利用NTsys和STRUCTURE软件进行聚类分析和群体遗传结构分析。【结果】9对SSR引物共扩增出78个条带,每个位点的等位标记数4~14个,平均每对引物为8.67个,多态信息含量变幅为0.58~0.82,平均为0.73。聚类分析显示辽东栎、蒙古栎分别聚为两个类群,猩红栎和北美红栎聚为一个类群,群体遗传结构分析显示23份种质为3个亚群体,遗传结构分析与聚类分析结果一致。【结论】23份栎属近缘种质可以划分为辽东栎组、蒙古栎组、猩红栎-北美红栎混合组,辽东栎与蒙古栎是两个独立的分类单位,上述结果为栎属分类、品种鉴定和知识产权保护提供了理论依据。[Objective] The fingerprint of the relative Quercus species was constructed and the genetic structure was analyzed,it provides an important tool for the identification and classification in relative Quercus species in order to classify and identify the relative Quercus species. [Method]23 germplasms of relative Quercus were used as the materials in this study,SSR primers were selected by 4 accessions of genetically distant germplasms,9 pairs of primers with clear amplification bands,high polymorphism and stable repeatability were selected and used for PCR amplification of the 23 germplasms,PCR products labeled fluorescent were detected using capillary electrophoresis technology,a strategy of combining primer pair with molecular weight for fingerprint construction was developed and applied to the 23 germplasms,the software of NTsys and STRUCTURE were used in clustering analysis and population genetic structure analysis. [Result] The 78 fragments were amplified by 9 pairs of SSR primers,the number of alleles at each locus was between 4 and 14,with an average of 8. 67 alleles for each pair ofprimers,polymorphism information content values for the primer pairs ranged from 0. 58 to 0. 82,with an average of 0. 73. Clustering analysis showed that Quercus liaotungensis and Quercus monglica were respectively divided into two groups,Quercus coccinea and Quercus rubra were clustered into a group,population genetic structure analysis showed that 23 germplasms for the three groups,genetic structure analysis and clustering analysis results were consistent. [Conclusion] The 23 germplasms are divided into Quercus liaotungensis group,Quercus monglica group,Quercus coccinea-Quercus rubra mixed group,Quercus liaotungensis and Quercus monglica are two separate taxonomies,the results provide a theoretical basis for Quercus classification,variety identification and protection of intellectual property rights.

关 键 词：辽东栎 蒙古栎 指纹图谱 遗传结构 

分 类 号：S718.46[农业科学—林学]