烟曲霉对人THP-1细胞中IL-6分泌和信号分子IκBα活化的影响  

Effect of Aspergillus fumigatus on the production of interleukin-6 and activation of IκBα in human THP-1 monocytes

作　　者：童建波 杜蕾蕾[1] 曾荣[1] 王丽玮[1] 刘宇甄 段志敏[1] 陈青[2] 李岷[1] TONG Jianbo;DU Leilei;ZENG Rong;WANG Liwei;LIU Yuzhen;DUAN Zhimin;CHEN Qing;LI Min(Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Jiangsu Key Laboratory of Molecular Biology for Skin Diseases, Nanjing 210042, China)

机构地区：[1]中国医学科学院&北京协和医学院皮肤病研究所江苏省皮肤性病学分子生物学重点实验室,南京210042 [2]江苏省血液中心,江苏南京210000

出　　处：《中国麻风皮肤病杂志》2018年第5期261-264,共4页China Journal of Leprosy and Skin Diseases

基　　金：中国医学科学院医学与健康科技创新工程重大项目(编号:2017-12M-1-017);973计划项目(编号:2013CB531600);江苏省科教强卫工程医学重点人才项目(编号:ZDRCB201610);国家自然科学基金青年项目(编号:81502739);江苏省自然科学基金青年项目(编号:BK20150068)

摘　　要：目的:明确烟曲霉对THP-1细胞中IL-6水平和细胞内信号分子NF-κB抑制蛋白(IκBα)激活的影响。方法:体外培养THP-1细胞,分为10~6CFU/mL和10~7CFU/mL烟曲霉组,β-Glucan(100g/L)阳性对照组和空白对照组,RT-PCR和ELISA检测各组IL-6 mRNA和蛋白表达水平,免疫印迹法检测IκBα和磷酸化IκBα水平。结果:10~6 CFU/mL IL-6 mRNA水平低于10~7CFU/mL烟曲霉组,选择10~7CFU/mL烟曲霉组作为实验浓度。10~7CFU/mL烟曲霉组IL-6 mRNA和蛋白浓度分别为(209.38±25.35)ng/L和(879.86±32.35)ng/L高于空白对照组的(1.19±0.36)和(10.67±0.17)ng/L,差异均有统计学意义(均P<0.05)。烟曲霉作用于THP-1细胞后磷酸化IκBα蛋白水平显著升高。NF-κB信号通路抑制剂BAY 11-7082可显著降低烟曲霉组IL-6 mRNA的表达。结论:烟曲霉可能通过激活THP-1细胞中的IκBα刺激IL-6分泌。Objective： To determine the effect of Aspergillus fumigatus on the production of interleukin-6 and activation of IκBα in an acute monocytic leukemia cell line THP-1. Methods： THP-l cells were cultured in vitro and divided into 10^6 CFU/mL and 10^7 CFU/mL A. fumigatus group,100 μg/mL β-Glucan control group and blank control group. The level of IL-6 mRNA and protein was detected by RT-PCR and ELISA respectively. The level of IκBα and phosphorylated IκBα was detected by Western blot. Results： The experimental concentration was selected to be 10^7 CFU/mL,because the level of IL-6 mRNA in the 10^6 CFU/mL A.fumigatus group was lower than that in the 10^7 CFU/mL group. The levels of IL-6 mRNA and protein in the 10^7 CFU/mL A. fumigatus group were 209.38±25.35 and 879.86±32.35 ng/L,which were higher than that in the blank control group（ 1.19±0.36 and 10.67±0.17 ng/L）,with significant differences（ Ps〈0.05）. The level of phosphorylated IκBα protein was increased significantly when induced by 10^7 CFU/mL A. fumigatus and was decreased when induced by NF-κB signaling pathway inhibitor of bay 11-7082. Conclusion： A. fumigatus stimulates the secretion of IL-6 in human THP-1 cells through activating IκBα.

关 键 词：烟曲霉 THP-1细胞系 IL-6 NF-κB 

分 类 号：R392[医药卫生—免疫学]