露天矿区人工林凋落物的木质纤维素酶活性和真菌多样性研究  

Study on Wood Cellulase Activity and Fungi Diversity of Artificial Forest Litter in Open Pit Mining Area

作　　者：王翔[1] 郭春燕[1] 王宇宏[1] 刘新志[1] 卢宁[1] 董娟 WANG Xiang;GOU Chunyan;WANG Yuhong;LIU Xinzhi;LU Ning;DONG Juan

机构地区：[1]山西省生物研究所,山西太原030006

出　　处：《山西科技》2018年第3期20-24,共5页Shanxi Science and Technology

基　　金：山西省应用基础研究项目;青年科技研究基金"露天煤矿人工林凋落物真菌多样性及木质纤维素酶活力的研究"(项目编号:201601D202063);自然科学基金"脆弱生态矿区人工林监测及健康评价研究"(项目编号:201701D121123);山西省科技基础条件平台项目"山西省植物标本资源共享服务平台"(项目编号:201605D121023)

摘　　要：使用传统酶学分析方法与分子生物学PCR-DGGE方法相结合,对安太堡露天矿区复垦地人工林凋落物的木质纤维素酶活性和真菌菌群的多样性进行了研究。结果表明,沙棘-刺槐-柠条-沙枣混交林下凋落物的锰过氧化物酶、羧甲基纤维素酶和滤纸酶的活性最高,青杄-白杄混交林凋落物的漆酶活性最高,不同组成的凋落物附生真菌群落多样性差异明显,真菌群落结构发生了显著变化。The cellulase activity and the diversity of the fungal flora of the litter in the reclaimed plantation of the opencast mining area should be study by using the traditional enzyme analysis method and the PCR-DGGE method of molecular biology. The results showed that the activity of manganese peroxidase,carboxymethyl cellulase and filter paper enzyme in the mixed forest under the mixed forest of Sea buckthorn-locust-Caragana-angustifolia was the highest,and the mixed forest of Picea wilsonii-Picea meyeri activity of the litter was the highest. The diversity of the epiphytic fungi in the litter was obvious,and the community structure of the fungi changed significantly.

关 键 词：凋落物 木质纤维素酶 真菌多样性 矿区人工林 

分 类 号：S718.8[农业科学—林学]