梭梭14-3-3蛋白基因HaFT-5、HaFT-6克隆及表达分析  

Cloning and Expression Analysis of HaFT-5 and HaFT-6 Genes in 14-3-3 Protein in Haloxylon ammodendron

作　　者：王娇[1] 王波 姜梦辉 李亚婕[1] 王泽 任燕萍[1] 张桦[1,2] Wang Jiao;Wang Bo;Jiang Menghui;Li Yajie;Wang Ze;Ren Yanping;Zhang Hua(College of Agronomy, Xinjiang Agricultural University, Urumqi, 830052;Institute of Desert in Drought Areas, Xinjiang Agricultural University, U- rumqi, 830052)

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]新疆农业大学干旱区荒漠研究所,乌鲁木齐830052

出　　处：《基因组学与应用生物学》2018年第5期2021-2028,共8页Genomics and Applied Biology

基　　金：国家自然科学基金(No.31260181);国家大学生创新项目(No.201610758056)共同资助

摘　　要：14-3-3蛋白是生物体内重要的调节蛋白,对细胞代谢及循环、信号传导、转录调节、蛋白跨膜转运、以及生物胁迫和非生物胁迫等多种生理过程均有重要的调节作用。本研究用RT-PCR从梭梭中克隆2个14-3-3蛋白基因,分别命名为Ha FT-5、Ha FT-6。用q RT-PCR分析Ha FT-5、Ha FT-6基因在不同处理后的表达。研究显示,Ha FT-5全长501 bp,编码139个氨基酸;Ha FT-6全长647 bp,编码226个氨基酸。在干旱、高盐、ABA、IAA处理后梭梭Ha FT-5和Ha FT-6基因后呈现不同的表达模式。综上所述,这2个基因可以响应逆境胁迫和2种激素信号,本研究可为进一步探索梭梭的抗逆分子机制提供了理论基础。14-3-3 protein is an important regulatory protein in organisms,which plays a significant regulatory role on cell metabolism circulation,signal transduction,transcription regulation,protein transmembrane transport,biotic abiotic stress and other physiological processes.This study used the RT-PCR to clone 2 genes of 14-3-3 protein from Haloxylon ammodendron,named Ha FT-5,Ha FT-6,respectively.And q RT-PCR was used to analyse the expression of Ha FT-5 and Ha FT-6 genes after different treatments.The results showed that the full-length of Ha FT-5 was 501 bp,encoding 139 amino acids;the full-length of Ha FT-6 was 647 bp,encoding 226 amino acids.Ha FT-5 and Ha FT-6 genes of Haloxylon ammodendron presented different expression patterns after drought,high salt,ABA and IAA treatments.Above all,these 2 genes could respond to stress and 2 hormone signals.The study might lay the foundation for further studies on molecular mechanism of stress resistance of Haloxylon ammodendron.

关 键 词：14-3-3蛋白 梭梭 基因克隆 表达分析 

分 类 号：Q943.2[生物学—植物学] S792.99[农业科学—林木遗传育种]