姜黄素对维生素D3和尼古丁诱导血管钙化大鼠血管中组织蛋白酶D表达的影响  

Effect of curcumin on the expression of cathepsin D in vascular calcification rats induced by Vitamin D3 and nicotine

作　　者：付尧[1] 吴冬冬 李晓东[1] FU Yao;WU Dong-Dong;LI Xiao-Dong(Department of Cardiology, Shen~ing Hospital of China Medical University, Shenyang , Liaoning 110001, China)

机构地区：[1]中国医科大学附属盛京医院心内科

出　　处：《中国动脉硬化杂志》2018年第5期485-490,共6页Chinese Journal of Arteriosclerosis

摘　　要：目的研究组织蛋白酶D（CTSD）在大鼠动脉钙化模型中的表达以及姜黄素对其表达的影响。方法将30只雄性SD大鼠随机分成3组,每组10只,即对照组、钙化组和干预组。对照组大鼠给予等量生理盐水处理,钙化组大鼠用维生素D3和尼古丁处理,干预组大鼠在钙化组处理的基础上加用姜黄素干预。用von Kossa染色检测血管钙化,试剂盒检测血管钙含量和碱性磷酸酶活性,Western blot检测骨形态发生蛋白2（BMP-2）、Runx2和CTSD的蛋白表达,用免疫组织化学染色观察CTSD在血管中的表达及分布。结果维生素D3和尼古丁能够诱导经典大鼠钙化模型,von Kossa染色结果显示钙化组大鼠胸主动脉有大量黑色颗粒沉积,血管钙含量和碱性磷酸酶活性升高,Western blot检测结果显示BMP-2和Runx2表达显著上调;使用姜黄素干预后,与钙化组相比,大鼠胸主动脉钙化程度明显减轻,血管钙含量和碱性磷酸酶活性下降,BMP-2和Runx2表达下调。免疫组织化学染色结果显示钙化组CTSD表达上调且大量分布在细胞外基质中,姜黄素能够显著下调CTSD的表达并减少其在细胞外基质中的分布。结论姜黄素具有抑制血管钙化的作用,可能与其能够减少CTSD在细胞外基质中的表达有关。Aim To study the protective effect of curcumin on vascular calcification in rat model. Methods 30 male SD rats were randomly divided into 3 groups,10 in each group,namely the control group,the calcification group and the intervention group. Vitamin D3 and nicotine were used to establish vascular calcification model. Vascular calcification was detected by von Kossa staining,calcium content and the activity of alkaline phosphatase（ ALP） were detected by kit,Western blot was used to detect the protein expression of bone morphogenetic protein-2（ BMP-2）,Runx2 and cathepsin D（ CTSD）,immunohistochemical staining was used to observe the expression and distribution of CTSD in blood vessels. Results Vitamin D3 and nicotine could induce vascular calcification,increase calcium content and the activity of ALP,and upregulate the expression of BMP-2 and Runx2. Meanwhile,curcumin could play a protective effect against the vascular calcification,decrease calcium content and the activity of ALP,and downregulate the expression of BMP-2 and Runx2 induced by Vitamin D3 and nicotine. Furthermore,CTSD was upregulated and distributed in extracellular matrix after treated with Vitamin D3 and nicotine. Curcumin could downregulate the expression of CTSD and reduce its distribution in extracellular matrix. Conclusion Curcumin could inhibit vascular calcification in rat model induced by vitamin D3 and nicotine and it may play protective role via regulating the expression of CTSD.

关 键 词：姜黄素 血管钙化 组织蛋白酶D 

分 类 号：R96[医药卫生—药理学]