Molecular Characterization and Correlation with β-lactam Resistance of Streptococcus pneumonia Isolates in Hangzhou,China  

作　　者：CHU Mei Fen LIU Xiao Xiang ZHANG Shao Ni HUANG Yan Ying2 3 DU Peng2 WANG Li Fang2 JI Lei2 YAN Jie4 5 SUN Ai Hua2 chu mei fen;liu xiao xiang;zhang shao ni;huang yan ying;du peng;wang li fang;ji lei;yan jie;sun ai hua

机构地区：[1]department of laboratory medicine,hangzhou medical college,Hangzhou 310053,Zhejiang,China [2]faculty of basic medicine,hangzhou medical college,Hangzhou 310053,Zhejiang,China [3]school of laboratory medical science and life science,wenzhou medical university,Wenzhou 325035,Zhejiang,China [4]division of basic medical microbiology,state key laboratory for diagnosis and treatment of infectious diseases,first affiliated hospital of zhejiang university,Hangzhou 310003,Zhejiang,China [5]department of medical microbiology and parasitology,college of medicine,zhejiang university,Hangzhou 310058,Zhejiang,China

出　　处：《Biomedical and Environmental Sciences》2018年第5期389-393,共5页生物医学与环境科学（英文版）

基　　金：supported by Grants from the National Natural Sciences Foundation of China[81772232];Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents

摘　　要：Penicillin-binding proteins（PBPs） are the target of β-lactam antibiotics（the major treatment for Streptococcus pneumoniae infections）,and mutations in PBPs are considered as a primary mechanism for the development of β-lactam resistance in S.pneumoniae.This study was conducted to investigate the mutations in the PBPs of clinical S.pneumoniae isolates in Hangzhou,China,in correlation with β-lactam resistance.Results showed that 19 F was the predominant serotype（7/27） and 14 of the S.pneumoniae isolates were resistant to both penicillin G and cephalosporin.Genotyping results suggested that β-lactam-resistant isolates primarily exhibited single-site mutations in both the STMK and SRNVP motifs of pbp1 a in combination with double-site mutations in the STMK motif of pbp2 x,which might be the primary mechanisms underlying the β-lactam resistance of the isolates in this study.Penicillin-binding proteins（PBPs） are the target of β-lactam antibiotics（the major treatment for Streptococcus pneumoniae infections）,and mutations in PBPs are considered as a primary mechanism for the development of β-lactam resistance in S.pneumoniae.This study was conducted to investigate the mutations in the PBPs of clinical S.pneumoniae isolates in Hangzhou,China,in correlation with β-lactam resistance.Results showed that 19 F was the predominant serotype（7/27） and 14 of the S.pneumoniae isolates were resistant to both penicillin G and cephalosporin.Genotyping results suggested that β-lactam-resistant isolates primarily exhibited single-site mutations in both the STMK and SRNVP motifs of pbp1 a in combination with double-site mutations in the STMK motif of pbp2 x,which might be the primary mechanisms underlying the β-lactam resistance of the isolates in this study.

关 键 词：PBP lactam Resistance of Streptococcus pneumonia Isolates in Hangzhou China RSP 

分 类 号：R446.5[医药卫生—诊断学]