钝挫伤及力竭运动对大鼠骨骼肌卫星细胞激活及Pax7/CBF1/DAPT含量的影响  

The Effect of Contusion and Exhaustive Exercise on Satellite Cells Activation and Pax7/CBF1/DAPT Contents in Skeletal Muscles of Rats

作　　者：潘同斌[1] 叶雷雷[2] 马兵[1] 李娜[1] 仝昕炜 汪亚如 Pan Tongbin;Ye Leilei;Ma Bing;Li Na;Tong Xinwei;Wang Yaru(College of Physical Education, Yangzhou University, Yangzhou 225009, China;Training division, Nanjing Institute of Physical Education and Sports. Nanjing 210014, china)

机构地区：[1]扬州大学体育学院,扬州225009 [2]南京体育学院训练处,南京210014

出　　处：《中国运动医学杂志》2018年第5期406-413,共8页Chinese Journal of Sports Medicine

基　　金：江苏省高校自然科学研究面上项目(15KJD310001)

摘　　要：目的:研究钝挫伤及力竭运动对大鼠骨骼肌卫星细胞激活及Pax7/CBF1/DAPT含量的影响,探索损伤修复的机制。方法:取7周龄雄性SD大鼠24只,分为4组,每组6只:安静对照组(C组);力竭运动即刻组(E0组);力竭运动后24 h组(E24组);力竭运动后48 h组(E48组)。另取18只SD大鼠,分为3组,每组6只:钝挫伤即刻组(Do组);钝挫伤后24 h组(D24组);钝挫伤后48 h组(D48组)。各组分别于安静状态、力竭运动后和钝挫伤后不同时间点,宰杀取血,分离血清。并取下右侧腓肠肌,将腓肠肌分为二份,一份立即进行细胞培养实验,另一份和血清一起在-80℃冰箱保存,用ELISA法检测Pax7、CBF1、DAPT的含量变化。另取新生3日龄鼠3只,宰杀后同样取右侧腓肠肌进行细胞培养实验。结果:(1)体外培养结果显示:新生鼠1天后即可见到梭形卫星细胞,其它各组的肌卫星细胞增长速度较慢,到第3天、第5天时各组开始大量增殖梭形细胞,且一直保持增殖良好并传代,第7天仍有较好的长势,且出现部分融合和微管。各组比较显示,安静对照组未见卫星细胞增殖;新生鼠卫星细胞数量最高,增殖速度也最快;而钝挫伤组的肌卫星细胞数量及增殖速度显著高于力竭运动组。(2)ELISA结果显示:力竭运动组和钝挫伤组各组骨骼肌、血清中CBF1和Pax7的含量与安静对照组相比均显著上调(P<0.05),其中D0组的CBF1含量差异极显著(P<0.01),明显高于其他各组(P<0.05);D24、D48两组的Pax7含量亦明显高于其他各组(P<0.05)。而DAPT在各组骨骼肌、血清中的含量与安静对照组相比均显著下调(P<0.05),其中D0组DAPT差异极显著(P<0.01)且明显低于其他各组(P<0.05)。但力竭运动各组(E0、E24、E48)之间无显著性差异。结论:(1)钝挫伤及力竭运动可使肌卫星细胞激活、增殖分化,并且钝挫伤导致的肌卫星细胞的激活数量明显高于力竭运动组,可能由于钝挫伤的刺激更强所致。(2)钝挫伤和Abtract Objective To study the effect of contusion and exhaustive exercise on satellite cells＇ acti-vation and Pax7/CBF1/DAPT contents in skeletal muscles of rats, and reveal the repair mechanism ofthe skeletal muscle injury. Methods Twenty-four seven-week-old male Sprague-Dawley（SD）rats wererandomly divided into a control（C）group, an immediately after exhaustive exercise（E0）group, a 24 hoursafter exhaustive exercise（E24）group and a 48 hours after exhaustive exercise（E48）group, each of 6.Other 18 SD rats were randomly divided into 3 groups, 6 rats in each group： an immediately after con-tusion group（D0）, a 24 h post-contusion group（D24）and a 48 h post-contusion group（D48）. All groupswere killed at different time points after exhaustive exercise and the contusion respectively while thecontrol group was at resting state, and their serum was extracted. The right gastrocnemius muscleswere resected and divided into 2 parts： one was used immediately for culturing satellite cells, whilethe other was stored in the bridge at-80℃. All the serum and gastrocnemius muscles were testedfor Pax7, CBF1 and DAPT contents using the enzyme-linked immunosorbent assay（ELISA）. Moreover,three-day-old newborn rats were also slaughtered for the above experiment. Results The results of cul-ture in vitro showed that one day after the culture began a small amount of spindle satellite cellscould be seen in the newborn rats, but the satellite cells of other groups were growing slowly. Howev-er, on the third day and the fifth day, the spindle satellite cells of each group began to proliferate inquantity and reached the peak. Then all the satellite cells had a good proliferation until the seventhday after serial passage, and some patial fusions to microtubules occurred. The biggest cell numberand proliferation rate was in newborn rats, but no skeletal muscle satellite cell was found in the con-trol group. At the same time, the number and the proliferation rate of skeletal muscle satellite cells incontu

关 键 词：钝挫伤 力竭运动 肌卫星细胞 Pax7 CBF1 DAPT 

分 类 号：R873[医药卫生—运动医学]