催化H_2O_2氧化吖啶黄固体基质室温磷光法测定痕量秋水仙碱  

ULTRA-SENSITIVE SOLID SUBSTRATE-ROOM TEMPERATURE PHOSPHORIMETRY FOR COLCHICINE DETECTION BASED ON ITS CATALYTIC EFFECT ON H_2O_2 OXIDIZE ACRIDINE YELLOW

作　　者：孙莉娜[1] 林常青[1] 郑志勇[1] SUN Li-na;LIN CHANG-qing;ZHENG Zhi-yong(College of Food Engineering, Zhangzhou Institute ofTeclmology, Zhangzhou, Fujian 363000, Chin)

机构地区：[1]漳州职业技术学院食品工程学院,福建漳州363000

出　　处：《井冈山大学学报（自然科学版）》2018年第2期23-30,共8页Journal of Jinggangshan University (Natural Science)

摘　　要：基于秋水仙碱(COL)能催化过氧化氢(H_2O_2)氧化吖啶黄(AY)的反应,导致AY的室温磷光信号(RTP)剧烈猝灭,据此建立了超灵敏催化H2O2氧化AY固体基质室温磷光法(SS-RTP)测定COL的新方法。此方法的量化限(LOQ)为0.12 fg/斑(对应浓度为3.1×10–13 g·m L–1),灵敏度高、简便、快捷、准确。用于血清中COL的测定,结果与UPLC-MS/MS相吻合。同时测定了动力学常数,其活化能(E)为40.53 k J·mol^(–1),速度反应常数(k)为3.97×10^(–4)·s^(–1)。A new solid substrate-room temperature phosphorimetry （SS-RTP） for colchicine （COL） detection has been established based on its strong catalytic effect on H2O2 oxidize acridine yellow（AY）, which caused the room temperature phosphorimetry （RTP） of AY to quench sharply. This high sensitive （limit of quantification （LOQ）： 3.1×10^-13 g·mL^-l）, accurate and selective SS-RTP has been successfully applied in the COL detection in the human serum and tea samples with the results agreeing well with high performance liquid chromatography （HPLC）, the results were coincident with those of ultra fast high performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） method. The activation energy and the reaction rate constant of catalytic reaction were 40.53 kJ·mol^-1 and 3.97×10^-4·s^-1, respectively.

关 键 词：秋水仙碱 吖啶黄 过氧化氢 磷光猝灭法 

分 类 号：O614.4[理学—无机化学]