IVIG对PMA诱导的人巨噬细胞吞噬功能的影响  

Effect of IVIG on phagocytosis of human macrophages induced by PMA

作　　者：侯明霞[1] 叶生亮[1] 刘凤娟[1] 李鸫 马莉[1] 李长清[1] HOU Mingxia;YE Shengliang;LIU Fengjuan;LI Dong;MA Li;LI Changqing.(Institute of Blood Transfusion, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Chengdu 610052, China.)

机构地区：[1]中国医学科学院北京协和医学院输血研究所

出　　处：《中国输血杂志》2018年第3期243-247,共5页Chinese Journal of Blood Transfusion

基　　金：2016四川省卫生和计划生育委员会科研课题(16ZD044);四川省科技厅计划项目(2017JY0064)

摘　　要：目的研究静脉注射人免疫球蛋白（intravenous immunoglobulin,IVIG）对佛波酯（phorbol 12-myristate 13-acetate,PMA）体外诱导分化成的人巨噬细胞吞噬功能的影响,探讨IVIG对自身免疫性疾病的免疫抑制作用。方法首先利用PMA体外诱导人急性单核白血病细胞THP-1分化形成巨噬细胞;然后利用流式细胞技术比较不同浓度的IVIG对巨噬细胞吞噬致敏红细胞的吞噬功能的影响。结果使用100 ng/m L PMA诱导THP-1细胞24 h,可成功诱导分化形成巨噬细胞。此外,当IVIG浓度为10μg/m L时,可完全抑制巨噬细胞对致敏红细胞的吞噬作用。结论IVIG可以有效地抑制巨噬细胞的吞噬功能,且抑制作用呈剂量依赖性。本研究可为进一步保障IVIG制品的质量奠定方法学基础。Objective To investigate the effect of intravenous immunoglobulin（ IVIG） on the phagocytosis of human macrophages induced by phorbol 12-myristate 13-acetate（ PMA）in vitro and discuss the immunosuppressive effects for autoimmune disease by IVIG. Methods Firstly,human acute monocytic leukemia THP-1 cells were induced to differentiate into macrophages by PMA. Then the effects of different concentrations of IVIG on the phagocytosis of the sensitized erythrocytes by the macrophages were compared by flow cytometry. Results The THP-1 cells were induced by 100 ng/m L PMA for 24 h and successfully differentiated into macrophages. In addition,the phagocytosis of sensitized erythrocytes by the macrophages was completely inhibited when the concentration of IVIG reached10 μg/m L. Conclusion IVIG can effectively inhibit the phagocytosis of macrophages in a dose-dependent manner. This study lay a solid foundation of methodology for improved quality control of IVIG products.

关 键 词：静脉注射免疫球蛋白 单核/巨噬细胞 致敏红细胞 吞噬 

分 类 号：R457.1[医药卫生—治疗学] TQ464.7[医药卫生—临床医学]