高盐抑制Min6细胞胰岛素分泌功能及其机制研究  

Inhibition of high-salt on insulin secretion in pancreas islet Min6 cells

作　　者：陆文杰 沈甫明 LU Wen-jie;SHEN Fu-ming(Department of Shanghai Tenth People＇s Hospital, Nanjing Medical University, Nanjing 210000;Department of Pharmacy, Shanghai Tenth People＇s Hospital, Shanghai 200072)

机构地区：[1]南京医科大学附属上海十院临床医学院,南京210000 [2]上海市第十人民医院药学部,上海200072

出　　处：《中南药学》2018年第5期629-632,共4页Central South Pharmacy

摘　　要：目的研究高盐环境能否影响胰岛Min6细胞的分泌功能,并探讨其可能机制。方法以50mmol·L^(-1)浓度高盐刺激Min6细胞,用酶联免疫吸附测定(ELISA)法测定Min6细胞胰岛素分泌功能,细胞活性检测试剂盒(cck8)检测Min6细胞增殖活性,Annexin V FITC/PI流式细胞术检测Min6细胞凋亡百分比,Western blot检测Min6细胞内B细胞淋巴瘤-2(bcl-2)蛋白的表达。结果与同渗透压甘露醇(100 mmol·L^(-1))相比,高盐环境(50 mmol·L^(-1))能抑制Min6细胞胰岛素的分泌,降低Min6细胞增殖活性,诱导Min6细胞凋亡,抑制细胞内bcl-2蛋白的表达。结论高盐环境减少胰岛Min6细胞胰岛素分泌,作用机制可能与细胞增殖活性降低、凋亡增加有关。Objective To investigate the inhibition of high-salt on insulin secretion in pancreas islet Min6 cells and its possible mechanism. Methods Min6 cells were stimulated by high-salt at 50 mmol·L^（-1）. Insulin secretion in Min6 cells was measured by enzyme-linked immuno sorbent assay （ELISA）, cell proliferation was measured by cell counting kit-8 （cck8）, cell apoptosis was measured by Annexin V FITC/PI flow cytometry, and expression of B-cellymphoma-2 （bcl-2） in Min6 cells was determined by Western blot. Results Compared with mannitol （100 mmol·L^（-1）） of the same osmotic pressure, high-salt （50 mmol·L^（-1）） reduced insulin secretion and proliferation of Min6 cells, induced the apoptosis of Min6 cells, and decreased the expression of bcl-2 in Min6 cells. Conclusion High-salt can reduce the insulin secretion in Min6 cells. The possible mechanism may be related to the down-regulation of cell proliferation and up-regulation of cell apoptosis.

关 键 词：MIN6细胞 高盐 胰岛素分泌 增殖 凋亡 

分 类 号：R587.1[医药卫生—内分泌]