姜黄素通过调控microRNA-7641及其靶基因p16抑制膀胱癌T24细胞增殖的机制研究  

Curcumin suppresses microRNA-7641-mediated regulation of p16 expression in bladder cancer

作　　者：吴雅莉[1] 鲁琼[2] 王洋波 徐冉[3] 原海燕[2] 屈健[2] 周艳钢[2] 唐甜甜[2] WU Ya-li;LU Qiong;WANG Yang-bo;XU Ran;YUAN Hai-yan;QU Jian;ZHOU Yan-gang;TANG Tian- tian(Department of Science and Education, Hunan Provincial Maternal and Child Health Care hospital, Changsha 410008;Department of Pharmacy, Second Xiangya Hospital, Central South University, Changsha 41 O011;Department of Urology, Second Xiangya Hospital, Central South University, Changsha 410011)

机构地区：[1]湖南省妇幼保健院科教部,长沙410008 [2]中南大学湘雅二医院药学部,长沙410011 [3]中南大学湘雅二医院泌尿外科,长沙410011

出　　处：《中南药学》2018年第5期641-645,共5页Central South Pharmacy

基　　金：湖南省自然科学基金(项目编号:2016JJ2072)

摘　　要：目的确定可能导致膀胱癌的mi RNAs,并研究其在姜黄素抗癌作用中的机制。方法对T24和SV-HUC-1细胞进行了芯片测序和q PCR分析。检测mi R-7641在含姜黄素或不含姜黄素的T24和SVHUC-1细胞株中的潜在致瘤性。并使用蛋白质印迹分析p16是mi R-7641在T24细胞中作用的重要靶基因。结果姜黄素诱导mi R-7641的表达下调及p16的上调,而p16是mi R-7641的转录后水平靶点之一,从而导致膀胱癌细胞凋亡增加。结论研究表明姜黄素可能是治疗无肌肉浸润性膀胱癌的临床治疗的潜在有效药物。Objective To identify the dys-regulated miRNAs in bladder cancer and examine their role in the anticancer effects of curcumin. Methods A microarray profiling and qPCR analysis were conducted in the T24 and SV-HUC-1 cell lines. We examined the potential tumorigenicity of miR-7641 in the T24 and SVHUC-1 cell lines with or without curcumin. Western blot analysis showed that p16 is a target of miR-7641 in T24 cells. Results Curcumin induces the downregulation of miR-7641 and subsequent upregulation of p16 which is a target of miR-7641 at the post-transcriptional level, which leads to the decreased invasion and increased apoptosis of bladder cancer cells. Conclusion The study shows that curcumin may be a candidate agent for the clinical management of non-muscle-invasive bladder cancer.

关 键 词：姜黄素 microRNA-7641 P16基因 膀胱癌 T24细胞 

分 类 号：R285[医药卫生—中药学]