泽泻总三萜超声辅助提取工艺优化  

Optimization of extraction process for total triterpenoids in Alisma orientalis (Sam.) Juzep. by response surface methodology

作　　者：石峰[1,2] 陈雨 张亚琴 马留辉[1,2] 窦明明 杨正明 陈兴福[1,2] SHI Feng;CHEN Yu;ZHANG Ya-qin;MA Liu-hui;DOU Ming-ming;YANG Zheng-ming;CHEN Xing-fu(Agronomy College of Sichuan Agricultural University, Chengdu, Sichuan 611130, China;Key Laboratory of Crop Physiology, Ecology and Cultivation in Southwest, Ministry of Agriculture, Chengdu, Sichuan 611130, China)

机构地区：[1]四川农业大学农学院,四川成都611130 [2]农业部西南作物生理生态与耕作重点实验室,四川成都611130

出　　处：《食品与机械》2018年第3期170-174,共5页Food and Machinery

基　　金：四川省"十二五育种攻关"项目(编号:2011NZ0098-12-01)

摘　　要：以提高泽泻总三萜的提取率为目的,在浸提温度、浸提时间、乙醇体积分数、料液比及超声时间的单因素基础上,采用四因素三水平Box-Behnken响应面试验设计,优选总三萜提取工艺。结果表明:最佳提取条件为乙醇体积分数70%、浸提时间46.00 min,浸提温度57.00℃,料液比1∶14(g/mL),超声时间30 min,该条件下总三萜提取得率为2.98%。The optimum conditions of ultrasonic extraction of total triterpenoid from leaves of Alisma orientalis（Sam.）Juzep.were investigated in this study.On the basis of single factor experiments,with yields of total triterpenoid as indexes,the three levels of four factors of Box-Behnken experimental design and response surface methodology was adopted to optimize extraction conditions by taking extraction temperature,ethanol concentration,extraction time and the ratio of material to solvent as factors.Results： Optimal extraction technology was as following,i.e.the ethanol concentration of 70%,the extracting for 46.00 min at 57.00 ℃,and the ratio of material to solvent was 1∶14（g/mL）,ultrasonic time for 30 min.Then the total triterpenes extraction rate reached 2.98% in this condition.

关 键 词：泽泻 总三萜 超声提取 

分 类 号：R284.2[医药卫生—中药学]