Facile Route to Enzyme Immobilization： Gloucose Oxidase Entrapped in Titania Under Mild Environmental Conditions and Consequent Electrochemical Sensor Response  

作　　者：YU Zhihui LIU Dandan LI Yunqiao 

机构地区：[1]Key Laboratory of Beijing on Regional Air Pollution Control, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, P. R. China [2]National Institute of Metrology, Beijing 100029, P. R. China

出　　处：《Chemical Research in Chinese Universities》2018年第3期490-494,共5页高等学校化学研究（英文版）

基　　金：Supported by the General Program of Science and Technology Development Project of Beijing Municipal Education Commission of China（No.KM201210005008）, the National Natural Science Foundation of China（No.21275014） and the Program for New Century Excellent Talents in University, China（No.NCET- 12 -0603）.

摘　　要：A facile one-step method to the immobilization of the combination of glucose oxidase（GOD） and catalase（CAT） in mesostructured TiO2 was proposed, The results obtained by transmission electron microspectroscopy and nitrogen adsorption-desorption analysis clearly show that the TiO2 mediated by the combination of GOD and CAT（CGC） has a large surface area and a narrow pore-size distribution. The CGC immobilized on mesostructured TiO2 exhibits direct electrochemistry and good electrocatalytic performance without any electron mediator.A facile one-step method to the immobilization of the combination of glucose oxidase（GOD） and catalase（CAT） in mesostructured TiO2 was proposed, The results obtained by transmission electron microspectroscopy and nitrogen adsorption-desorption analysis clearly show that the TiO2 mediated by the combination of GOD and CAT（CGC） has a large surface area and a narrow pore-size distribution. The CGC immobilized on mesostructured TiO2 exhibits direct electrochemistry and good electrocatalytic performance without any electron mediator.

关 键 词：Direct electrochemistry Glucose oxidase IMMOBILIZATION 

分 类 号：O625.463[理学—有机化学] TS664.05[理学—化学]