三氯生联合纳米银用于增强抗菌效果的研究  

Triclosan combined with silver nanoparticle for enhanced antibacterial efficacy

作　　者：杨丽军[1] 王婧[1] 黄帆[1] 任春华[1] 禇丽萍 YANG Li-jun;WANG Jing;HUANG Fan;REN Chun-hua;CHU Li-ping(Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China)

机构地区：[1]北京协和医学院·中国医学科学院 放射医学研究所、天津市放射医学与分子核医学重点实验室,天津300192

出　　处：《生物医学工程与临床》2018年第3期251-256,共6页Biomedical Engineering and Clinical Medicine

基　　金：国家自然科学基金资助项目(51603231;21708048);中国医学科学院医学与健康科技创新工程项目(2016-I2M-3-022;2017-I2M-2-004);天津市自然科学基金资助项目(16JCQNJC02500);“药物毒理与放射损伤药物山西省重点实验室”开放课题(ZFKF-002)

摘　　要：目的设计合成一类新型的三氯生-纳米银(TCS-Ag NPs)联合抗菌剂,对其理化性质进行表征,并研究其体外抗菌活性。方法通过半胱氨酸(Cys)上的巯基将甘氨酸-甘氨酸-半胱氨酸(Gly-Gly-Cys)三肽修饰的三氯生(TCS)连接到纳米银(Ag NPs)上,得到TCS-Ag NPs。利用核磁氢谱(~1H-NMR)和高分辨质谱(HRMS)对三氯生-戊二酸酐-甘氨酸-甘氨酸-半胱氨酸(TCS-GA-Gly-Gly-Cys)进行结构确证;通过动态光散射(DLS)和透射电子显微镜(TEM)分析纳米粒的粒径和形貌;通过紫外-可见吸收光谱表征纳米粒的光学性质;采用扫描电子显微镜(SEM)和全波长多功能酶标仪分别定性和定量地研究TCS-Ag NPs对金黄色葡萄球菌的增殖抑制效果。结果 TCS-Ag NPs粒径为45 nm左右的球形纳米粒。TCS-Ag NPs的ζ-电位为(-26.6±1.9)mV。TCS-Ag NPs对金黄色葡萄球菌的增殖抑制呈现质量浓度依赖性,在0.5~20.0μg/mL的Ag质量浓度及0.4~16.0μg/mL的TCS质量浓度范围内,其抑菌效果均好于Ag NPs和游离TCS。结论TCS-Ag NPs是一种有效的纳米抗生素,能发挥广谱抗菌剂Ag NPs和抗生素TCS的协同抗菌效果,对细菌进行联合杀伤。Objective To design and synthesize a novel combination antibacterial triclosan-silver nanoparticle（TCS-Ag NPs） and characterize the physical and chemical properties, and investigate antibacterial activity in vitro. Methods The TCS-Ag NPs was prepared by attaching tripeptide（Gly-Gly-Cys）-modified triclosan（TCS） to silver nanoparticles（Ag NPs） via sulfhydryl group on eysteine（Cys）. The structure of TCS-GA-Gly-Gly-Cys was confirmed by hydrogen nuclear magnetic resonanee（1H- NMR） and high resolution mass spectrometry（HRMS）. The particle size and micromorphology of the nanoparticles were detected by dynamic light scattering（DLS） and transmission electron microscopy（TEM）, respectively. The optical properties of the nanopar- ticles were characterized by UV-vis absorption spectroscopy. The antibacterial efficacy of TCS-Ag NPs against S. aureus was qualitatively and quantitatively evaluated by scanning electron microscopy（SEM） and full-wavelength multi-function microplate reader, respectively. Results The TCS-Ag NPs were spherical nanoparticles with size about 45 nm. The E-potential of TCS- Ag NPs was （- 26.6± 1.9） mV. TCS-Ag NPs significantly inhibited the proliferation of S. aureus and the bacteria viability was obviously concentration-dependent. At each concentration（0.5 - 20.0 μg/mL Ag and 0.4 - 16.0 μg/mL TCS）, TCS-Ag NPs showed the highest antibacterial activity. Conclusion It is demonstrated that TCS-Ag NPs is an effective nanoantibiotics, which could inhibit bacteria growth on account of the synergistic antibacterial effect of both broad spectrum antibacterial Ag NPs and antibiotic TCS.

关 键 词：三氯生 纳米银 纳米抗生素 联合治疗 抗菌 

分 类 号：R318.08[医药卫生—生物医学工程]