新西兰兔颅骨缺损钛网植入后钛离子释放的研究  

Titanium ion release in titanium plate implanted skull defect rabbit

作　　者：王蕾 张毅[2] 严红燕[2] 彭强 堵俊[3] WANG Lei;ZHANG Yi;YAN Hong-yan;PENG Qiang;DU Jun(a Department of Emergeney Center, b. Department of Nettrosurgery, Second Affiliated Hospital of Nantong University, Narttong 226001, Jiangsu, China;School of Electrical Engineering, Nantong University, Nantong 226019, Jiangsu, China)

机构地区：[1]南通大学第二附属医院急诊中心,江苏南通226001 [2]南通大学第二附属医院神经外科,江苏南通226001 [3]南通大学电气工程学院,江苏南通226019

出　　处：《生物医学工程与临床》2018年第3期257-261,共5页Biomedical Engineering and Clinical Medicine

基　　金：南通市科技计划项目(MS12015109)

摘　　要：目的以颅骨缺损植入钛网的新西兰兔为观察对象,检测钛离子在体内的释放及变化趋势,为钛金属在临床安全应用提供实验依据。方法选择健康新西兰兔48只,雌雄各半,兔龄3~4个月,体质量2.5~3.5 kg。制作颅脑缺损模型,分为对照组和实验组。对照组24只,均未植入钛修复体;实验组24只,行钛网修补,随机分为4组(依据术后1、3、6、12个月划分),每组6只。采用电感耦合等离子体质谱分析仪(ICP-MS)测定血清、尿液、毛发及肝、脾、肺、肾、脊髓等钛离子浓度,对相关临床资料进行比较。结果钛植入体术后血清和尿液中钛离子浓度较术前明显升高,术后1~6个月与术前比较,差异有统计学意义(P<0.05),其中血清钛离子浓度[(87.13±10.51)ng/mL]在术后第3个月达到最高,尿液钛离子浓度[(68.32±18.22)ng/mL]在术后第1个月达到最高,随后下降并均在术后第12月钛离子水平逐渐接近术前,差异无统计学意义(P>0.05);术后肺、肝、肾、脾、脊髓、淋巴结中钛离子水平较对照组有明显升高,差异有统计学意义(P<0.05)。术后1个月内脏组织器官钛离子浓度均达到最高值,术后有下降趋势。其中脊髓的钛离子浓度在术后1、3、6、12个月[(3.64±0.15)μg/mL、(2.43±0.17)μg/mL、(1.87±0.34)μg/mL、(1.76±0.19)μg/mL]均高于其他内脏组织。结论钛植入体在新西兰兔体内是相对安全的,远期需要建立一个人体相对安全的钛离子浓度检测体系。Objective To observe the release and change trend of titanium ion in rabbit with skull defect implanted titanium plate, and provide experimental basis for clinical application safety of titanium metal. Methods Forty-eight healthy rabbits were selected, half male and half female, rabbit aged 3 - 4 months old with body weight of 2.5 - 3.5 kg. The skull defect models were divided into control group（n = 24, without any titanium restorer） and experimental group（n = 24）. The experimen- tal group was randomly divided into 4 sub-group by implantation time（n = 6, 1, 3, 6, 12 months after operation）. The induc- tively coupled plasma mass spectrometry（ICP-MS） was used to determine the concentration of titanium ion in serum, urine, hair and liver, spleen, lung, kidney and spinal cord, and the relevant clinical data was compared. Results The concentration of titanium ion in serum and urine after titanium implantation was significantly higher than that of pre-implantation, and there was statistical difference between data of before implantation and 1 - 6 month after implantation（P 〈 0.05）. The titanium ion concentration in serum reached the peak 3-month after implantation[（87.13 ± 10.51） ng/mL]. After implantation for one- month, titanium ion concentration of urine reached the peak[（68.32 ±18.22） ng/mL], and then decreased and gradually dropped to preoperafion level at 12-month after implantation, and the difference was no statistically significant（P 〉 0.05）. The titanium ion in lung, liver, kidney, spleen, spinal cord and lymph nodes of experimental group was significant higher than that of control group, and the difference was statistically significant（P 〈 0.05）. One month after implantation, the concentration of titanium ions in visceral tissues reached the peak and then declined. The titanium ion concentration of spinal cord was higher than that in other intemal organs at 1-month[（3.64 ± 0.15） μg/mL], 3-month[（2.43 ± 0.17） μg/mL], 6-month[（1.87 ± 0.34） μg/mL] an

关 键 词：钛离子 体内释放 钛离子分布 血液浓度 颅骨修补 兔 

分 类 号：R318.08[医药卫生—生物医学工程]