13种兽用抗菌药对大肠杆菌及沙门菌的体外抑菌效果  

Antibacterial effects of 13 antibacterials on Escherichia coli and Salmonella strains

作　　者：王虹[1] 杨德鸿 汤芳[1] 李亚芯[1] 徐长青 胡贺 张永春 任建鸾[1] WANG Hong;YANG Dehong;TANG Fang;LI Yaxin;XU Changqing;HU He;ZHANG Yongchun;REN Jianluan(Key Laboratory of Animal Bacteriology of Ministry of Agriculture/College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;Nanjing Furunde Pharmaceutical Company Limited, Nanjing 210007, China)

机构地区：[1]南京农业大学动物医学院/农业部动物细菌病重点实验室,江苏南京210095 [2]南京福润德动物药业有限公司,江苏南京210007

出　　处：《畜牧与兽医》2018年第6期110-113,共4页Animal Husbandry & Veterinary Medicine

基　　金：国家重点研发计划项目(2016YFD0500800)

摘　　要：为了解目前市售抗菌药对畜牧生产中常见病原菌的抑菌作用,选用13种常见抗菌药,对参考菌株大肠杆菌（ATCC25922）进行了最小抑菌浓度（MIC）测定,并挑选其中抑菌效果相对较好的药物对来自河南省和江苏省各集约化禽类养殖场分离鉴定所得的39株大肠杆菌和沙门菌进行了MIC测定。结果显示：药物D（10%阿莫西林）、药物G（100 g∶10 g硫酸黏菌素）、药物I（100 mL∶25 g磺胺嘧啶、5 g甲氧苄啶）、药物J（100 mL∶磺胺间甲氧嘧啶钠10 g、甲氧苄啶2 g）、药物L（100 mL∶10 g氟苯尼考）抑菌效果较好（MIC〈78.12μg/mL）,药物E（5%盐酸多西环素）、药物F（10%盐酸多西环素）的抑菌效果次之（MIC为156.25μg/mL）,药物B（100 g∶3.25 g硫酸新霉素）和K（5 g戊二醛、5 g葵甲溴铵）的抑菌效果较差（MIC〈625μg/mL）,药物A（10%盐酸林可霉素）、药物C（100 g∶10 g A型酒石酸泰乐菌素）、药物H（100 g∶5 g硫氰酸红霉素）、药物M（聚维酮碘）抑菌效果最差,所有稀释倍数均长菌;挑选的抑菌效果较好的药物MIC结果显示,药物I对39个临床菌株均无效,药物L对5株临床菌株有效,药物E对23株临床菌株有效。结果表明：药物E可用于大肠杆菌、沙门菌感染的抗菌治疗。In order to investigate antibacterial effect of antibacterial drugs on the market on common pathogens in animal husbandry,minimal inhibitory concentrations（ MIC） of 13 kinds of antibacterial drugs against two kinds of pathogens（ Escherichia coli,and Salmonella）were tested,which were isolated on the farms in Henan and Jiangsu provinces. The results showed that antibacterial drugs D（ 10% of amoxicillin）,G（ 100 g ∶ 10 g colistin sulfate）,I（ 100 mL ∶ 25 g sulfadiazine and 5 g trimethoprim）,J（ 100 mL ∶ 10 g sulfadiazine sodium and2 g trimethoprim） and L（ 100 mL ∶ 10 g florfenicol） performed well（ MIC78. 12 μg/mL）; followed by E（ 5% of doxycycline HCl） and F（ 10% of doxycycline HCl）,with MIC of 156. 25 μg/mL; B（ 100 g ∶ 3. 25 g neomycin sulfate） and K（ 5 g glutaraldehyde and 5 g kwai methyl bromide） had even lower antibacterial effect（ MIC 625 μg/mL）; A（ 10% of lincomycin hydrochloride）,C（ 100 g ∶ 10 g of tylosin tartrate A）,H（ 100 g ∶ 5 gerythromycinthiocyanate） and M（ povidone iodine） showed the worst antibacterial effect,with undetectable MIC. I,L and E were tested against the 39 clinical strains. The results showed that I had no effect on all of the 39 strains,while L and E had effect against 5 and 23 strains,respectively. The results suggested that E could be used to be against infection of E. coli and Salmonella.

关 键 词：抗菌药 最小抑菌浓度(MIC) 耐药性 大肠杆菌 沙门菌 

分 类 号：S852.6[农业科学—基础兽医学]