四逆汤对实验性高脂血症合并动脉粥样硬化兔SDF-1、VEGF的影响  

Effect of Sini decoction on stromal cell-derived factor-1 and vascular endothelial growth factor in rabbits with experimental hyperlipidemia complicated by atherosclerosis

作　　者：黄振[1] 郁保生[1] 雎世聪 王元红[1] 邹旭峰[1] HUANG Zhen;YU Bao - sheng;JU Shi - cong;WANG Yuan - hong;ZOU Xu - feng(Hunan University of Chinese Medicine, Changsha 410208, Hunan, China)

机构地区：[1]湖南中医药大学,湖南长沙410208

出　　处：《湖南中医杂志》2018年第5期174-176,共3页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南省中医药管理局资助项目(编号:201518);湖南省学位与研究生教育教学改革研究课题(编号:JG2015B069);湖南中医药大学学位与研究生教育教学改革研究课题(编号:2013JG04)

摘　　要：目的:观察四逆汤对高脂血症(HLP)动脉粥样硬化(AS)兔血清血管内皮生长因子(VEGF)、基质细胞衍生因子1(SDF-1)表达的影响。方法:新西兰大耳白兔60只,随机分为6组,每组10只,即空白对照组(A组)、AS模型组(B组)、阿托伐他汀组(C组)、四逆汤低剂量组(D组)、四逆汤中剂量组(E组)、四逆汤高剂量组(F组)。兔HLP合并AS模型的建立采用高脂饲料喂养法,C、D、E、F组分别给予阿托伐他汀和四逆汤(高、中、低剂量)干预。12周后行主动脉切片HE染色观察,并检测所取兔血中VEGF和SDF-1水平。结果:与模型组比较,四逆汤高、中剂量组家兔血清VEGF和SDF-1水平降低,差异均有统计学意义(P<0.01)。结论:四逆汤可能对HLP合并AS兔的血管内皮有保护作用,从而降低VEGF和SDF-1水平。Objective： To investigate the effect of Sini decoction on the expression of vascular endothelial growth factor（ VEGF） and stromal cell-derived factor-1（ SDF-1） in serum in rabbits with hyperlipidemia（ HLP） complicated by atherosclerosis（ AS）. Methods： A total of 60 New Zealand big-eared white rabbits were randomly divided into blank control group（ group A）,AS model group（ group B）,atorvastatin group（ group C）,low-dose Sini decoction group（ group D）,middle-dose Sini decoction group（ group E）,and high-dose Sini decoction group（ group F）,with 10 rabbits in each group. The rabbits were given high-fat feed to establish a model of HLP complicated by AS,and the rabbits in groups C,D,E,and F were treated with atorvastatin and high-,middle-,and low-dose Sini decoction,respectively. After 12 weeks of intervention,HE staining was performed for aortic sections,and blood samples were collected to measure the serum levels of VEGF and SDF-1. Results： Compared with the model group,the high-and middle-dose Sini decoction groups had significant reductions in the serum levels of VEGF and SDF-1（ P〈0. 01）. Conclusion： Sini decoction has a protective effect on the vascular endothelium and can reduce the levels of VEGF and SDF-1 in rabbits with HLP complicated by AS.

关 键 词：高脂血症 动脉粥样硬化 四逆汤 血管内皮生长因子 基质细胞衍生因子1 实验研究 

分 类 号：R285.5[医药卫生—中药学]