内蒙古自治区巴彦淖尔地区鼠类携带疏螺旋体调查研究  

Survey on Borrelia bacteria in rodents from Bayannur,Inner Mongolia Autonomous Region, China

作　　者：乌兰图雅 高娃 殷旭红 曹民治 郭生春 王强 阿日奔吉日嘎拉 刘美琴[3] Wulantuya;Gaowa;YIN Xu-hong;CAO Min-zhi;GUO Sheng-chun;WANG Qiang;Arionjergal;LIU Mei-qin(Inner Mongolia Key Laboratory of Tick-borne Zoonotic Infectious Disease, Department of Medicine, Hetao College, Bayannur 015000, Inner Mongolia Autonomous Region, China;Bayannur Center for Disease Control and Prevention;Bayannur Hospital)

机构地区：[1]内蒙古自治区蜱媒人畜共患传染病重点实验室河套学院医学系,内蒙古巴彦淖尔015000 [2]巴彦淖尔市疾病预防控制中心,内蒙古巴彦淖尔015000 [3]巴彦淖尔市医院,内蒙古巴彦淖尔015000

出　　处：《中国媒介生物学及控制杂志》2018年第3期239-241,共3页Chinese Journal of Vector Biology and Control

基　　金：国家自然科学基金(31660044);2016年巴彦淖尔市科技计划项目~~

摘　　要：目的了解内蒙古自治区(内蒙古)巴彦淖尔地区鼠类分布、疏螺旋体感染及其基因型等,为该地疫情防控和风险评估提供基础数据。方法于2015-2016年每年的4-6月、9-10月采用夹夜法捕获鼠类,采用特异性PCR扩增法对标本进行疏螺旋体检测,并通过测序和序列比对等进行疏螺旋体基因型分析。结果共捕获鼠类480只,其中子午沙鼠(174只)和长爪沙鼠(124只)数量居多,为该地优势鼠种。鼠类脾脏标本经PCR检测,共发现8份疏螺旋体fla B和16S rDNA基因阳性标本,阳性率为1.67%,其中毛足鼠和长爪沙鼠的阳性率分别为7.14%(2/28)和4.84%(6/124)。对阳性标本基因片段进行序列分析发现,感染的疏螺旋体为Borrelia afzelii和B.garinii。结论内蒙古巴彦淖尔地区鼠类携带的疏螺旋体以B.afzelii和B.garinii为主,应加强该病原体的检测和莱姆病防制。Objective To examine the infection rate and genotype of Borrelia sp. among rodents, we surveyed rodent samples from Bayannur region of Inner Mongolia Autonomous Region, and to provide basic information of epidemic prevention and risk assessment for local area. Methods During 2015-2016, rodent samples were collected in Bayannur region by the trap-night method, and rodent spleen DNA samples were examined using specific PCR to detect the Borrelia sp. Results A total of 480 rodents were collected from Bayannur, and Meriones meridianus accounted for 174, and M. unguiculatus 124. The results showed that both were the predominant species in these areas. Borrelia sp. were identified from 8（ 1.67%） when amplified by PCR targeting flaB gene and 16S rDNA, including 6 cases of M. unguiculatus and 2 cases of Phodopus sungorus, the positive rate were 7.14% and 4.84%, respectively. Sequence analysis of the amplification products showed identification of B. garinii and B. afzelii with 100% homology, respectively. Conclusion In this study, we confirmed the B. garinii and B. afzelii were main pathogens transmitting among rodents in Bayannur region, it is necessary to implement monitoring and prevention of Lyme diseases.

关 键 词：鼠类 疏螺旋体 莱姆病 回归热 

分 类 号：R377[医药卫生—病原生物学]