无特定病原体麻鸭种群的建立  

Establish of Specific Pathogen-free Shedldrake duck Breed Populations

作　　者：韩凌霞[1] 赵丽丽[1] 于海波[1] 张伟[1] 张圆圆[1] 陈洪岩[1] HAN Lingxia;ZHAO Lili;YU Haibo;ZHANG Wei;ZHANG Yuanyuan;CHEN Hongyan(Division of Laboratory Animal and Comparative Medicine of Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Heilongfiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin 150001, China)

机构地区：[1]中国农业科学院哈尔滨兽医研究所实验动物与比较医学团队/国家禽类实验动物种子中心,哈尔滨150001

出　　处：《实验动物科学》2018年第2期56-59,64,共5页Laboratory Animal Science

基　　金：黑龙江省自然科学基金重点项目(No.ZD2016006);中央级公益性科研院所基本科研业务费专项(No.1610302017013;No.1610302018013)

摘　　要：目的建立无特定病原体麻鸭种群。方法以优质培育品种绍兴麻鸭白壳I号和金定鸭种卵为基础,通过疫病监测淘汰抗体抗原阳性个体、灭活疫苗免疫、整个生命周期应用鸭正压隔离器饲养、60Co射线辐照饲料、酸化饮用水、严格控制人流、物流等净化措施,同时开展了多种分子遗传学检测、生物学特性数据测定和疫病敏感性试验,以实现实验动物化。结果成功建立了无特定病原、遗传学背景清晰、疫病敏感的HBK-SPF和SPF-JD鸭种群。结论 SPF麻鸭种群丰富了我国实验动物资源,为生命科学研究和生物制品研发提供了重要条件支撑。Objective Establish specific pathogen-free Anas platyrhyncha var. domestica breed populations.Method Highly quality breed species of Shaoxing Shedldrake No. I and Jinding duck breed eggs were based on as original populations,various biological purification measures were adopted,including eliminated specific avian diseases antibodies and antigen positive individuals,immunized with killed vaccines,raised in positive pressure high efficiency particulate air filter,fed with 60 Co-treated feed,acidified water,strictly controlled the flow of people and logistics. Simultaneously,tests of molecular genetics,biological characteristics and disease susceptibility were carried out to standardize the ducks. Result HBK-SPF and SPF-JD duck breed populations with specific pathogens-free,clear genetics background and disease susceptible were successfully established. Conclusion The established specific pathogen-free duck breeds will enrich Chinese laboratory animals resources and provide important support for life science research and development of biological products.

关 键 词：麻鸭 无特定病原体 种群 

分 类 号：Q95-331[生物学—动物学]