ATP生物荧光法在洗手效果评价中的应用  

Application of ATP bioluminescence method used in hand washing effect evaluation

作　　者：许慧琼[1] 王一梅[1] 朱军生[1] 王斌[1] 刘小丽[1] 龚林[1] 梁建生[1] XU Hui - qiong;WANG Yi - mei;ZHU Jun - sheng;WANG Bin;LIU Xiao - li;GONG Lin;LIANG Jian - sheng(Wuhan Center for Disease Control and Prevention,Wuhan Hubei 430015, China)

机构地区：[1]武汉市疾病预防控制中心,湖北武汉430015

出　　处：《中国消毒学杂志》2018年第5期370-372,共3页Chinese Journal of Disinfection

基　　金：武汉市卫生计生委医疗卫生科研项目(WG15D08)

摘　　要：目的研究ATP生物荧光法在洗手效果评价中的应用。方法采用ATP生物荧光法,对武汉市部分医疗机构医务人员洗手效果进行评价,同时与菌落计数法作平行比较。结果编号A、B、C等3种ATP生物荧光检测仪分别检测108例、96例、108例医务人员洗手效果,洗手后RLU值下降率中位数依次为96.46%、94.34%和96.61%;菌落计数法检测洗手后细菌菌落数下降率中位数为99.61%。经Wilcoxon符号秩和检验,各种仪器/方法洗手后RLU值/细菌菌落数的下降率差异均无统计学意义;经Kruskal-Wallis H秩和检验,不同岗位工作人员洗手后RLU值/细菌菌落数的下降率之间差异也无统计学意义。结论采用ATP生物荧光法来评价洗手效果具有可行性,本研究推荐洗手后ATP生物荧光法检测RLU值下降率≥90%作为评价标准。Objective To study the application of ATP bioluminescence method used in hand washing effect evaluation.Methods Three kinds of ATP bioluminescence detectors were used to detect the effect of staff＇s hand washing,with colony counting method as parallel comparison. Results Three kinds of ATP bioluminescence detectors were used to detect the effect of 108,96,108 staff＇s hand washing,respectively. The median of the rate-of-descent of three detectors were 96.46%,94. 34%,96. 61% respectively,and the median of the rate-of-descent of cfu was 99. 61%. By Wilcoxon signed-rank test,the rate-of-descent of RLU/cfu between each two detectors/methods had no statistical significance. By Kruskal-Wallis H rank sum test,the rate-of-descent of RLU/cfu among different positions had no statistical significance.Conclusion It is feasible to evaluate the effect of hand washing by ATP bioluminescence method. And in this study,it is recommended that the rate-of-descent of RLU after hand washing which is equal and above 90% can be used as the evaluation standard for ATP bioluminescence method.

关 键 词：生物荧光法 菌落计数法 洗手 相对光单位 

分 类 号：R187[医药卫生—流行病学]